The Aux/IAA protein TaIAA15-1A confers drought tolerance in Brachypodium by regulating abscisic acid signal pathway.

IF 5.3 2区 生物学 Q1 PLANT SCIENCES
Peisen Su, Chao Sui, Jingyu Li, Kun Wan, Haonan Sun, Shuhan Wang, Xiaoqian Liu, Shangjing Guo
{"title":"The Aux/IAA protein TaIAA15-1A confers drought tolerance in Brachypodium by regulating abscisic acid signal pathway.","authors":"Peisen Su,&nbsp;Chao Sui,&nbsp;Jingyu Li,&nbsp;Kun Wan,&nbsp;Haonan Sun,&nbsp;Shuhan Wang,&nbsp;Xiaoqian Liu,&nbsp;Shangjing Guo","doi":"10.1007/s00299-022-02965-9","DOIUrl":null,"url":null,"abstract":"<p><strong>Key message: </strong>Overexpression of the Aux/IAA protein TaIAA15-1A from wheat improves drought tolerance by regulating the ABA signalling pathway in transgenic Brachypodium. Drought is a major abiotic stress that causes severe crop yield loss. Aux/IAA genes have been shown to be involved in drought stress responses. However, to the best of our knowledge, there has been little research on the molecular mechanism of the wheat Aux/IAA gene in the context of drought tolerance. In this study, we found that expression of the wheat Aux/IAA gene TaIAA15-1A was upregulated by PEG6000, NaCl, SA, JA, IAA and ABA. Transgenic plants overexpressing TaIAA15-1A showed higher drought tolerance than wild-type (WT) plants. The physiological analyses showed that the transgenic lines exhibited a higher survival rate, shoot length, and relative water content than the WT plants. The activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were enhanced in transgenic lines, causing a reduction in the hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and superoxide anion radical (O<sub>2</sub><sup>-</sup>) contents. Transcriptome analysis showed that TaIAA15-1A overexpression alters the expression of these genes involved in the auxin signalling pathway, ABA signalling pathway, phenolamides and antioxidant pathways. The results of exogenous ABA treatment suggested that TaIAA15-1A overexpression increased sensitivity to ABA at the germination and postgermination stages compared to WT plants. These results indicate that TaIAA15-1A plays a positive role in plant drought tolerance by regulating ABA-related genes and improving antioxidative stress ability and has potential application in genetically modified crops.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"42 2","pages":"385-394"},"PeriodicalIF":5.3000,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Cell Reports","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00299-022-02965-9","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 3

Abstract

Key message: Overexpression of the Aux/IAA protein TaIAA15-1A from wheat improves drought tolerance by regulating the ABA signalling pathway in transgenic Brachypodium. Drought is a major abiotic stress that causes severe crop yield loss. Aux/IAA genes have been shown to be involved in drought stress responses. However, to the best of our knowledge, there has been little research on the molecular mechanism of the wheat Aux/IAA gene in the context of drought tolerance. In this study, we found that expression of the wheat Aux/IAA gene TaIAA15-1A was upregulated by PEG6000, NaCl, SA, JA, IAA and ABA. Transgenic plants overexpressing TaIAA15-1A showed higher drought tolerance than wild-type (WT) plants. The physiological analyses showed that the transgenic lines exhibited a higher survival rate, shoot length, and relative water content than the WT plants. The activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were enhanced in transgenic lines, causing a reduction in the hydrogen peroxide (H2O2) and superoxide anion radical (O2-) contents. Transcriptome analysis showed that TaIAA15-1A overexpression alters the expression of these genes involved in the auxin signalling pathway, ABA signalling pathway, phenolamides and antioxidant pathways. The results of exogenous ABA treatment suggested that TaIAA15-1A overexpression increased sensitivity to ABA at the germination and postgermination stages compared to WT plants. These results indicate that TaIAA15-1A plays a positive role in plant drought tolerance by regulating ABA-related genes and improving antioxidative stress ability and has potential application in genetically modified crops.

Aux/IAA蛋白taaa15 - 1a通过调控脱落酸信号通路,赋予短柄植物抗旱性。
关键信息:小麦过表达Aux/IAA蛋白TaIAA15-1A,通过调控ABA信号通路提高小麦的抗旱性。干旱是造成农作物严重减产的主要非生物胁迫。Aux/IAA基因已被证明与干旱胁迫反应有关。然而,就我们所知,关于小麦Aux/IAA基因在耐旱性方面的分子机制的研究很少。在本研究中,我们发现PEG6000、NaCl、SA、JA、IAA和ABA上调了小麦Aux/IAA基因TaIAA15-1A的表达。过表达taaa15 - 1a的转基因植株比野生型(WT)植株具有更高的耐旱性。生理分析表明,转基因植株的成活率、茎长和相对含水量均高于野生型植株。转基因植株超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性增强,过氧化氢(H2O2)和超氧化物阴离子自由基(O2-)含量降低。转录组分析表明,TaIAA15-1A过表达改变了生长素信号通路、ABA信号通路、酚酰胺和抗氧化通路相关基因的表达。外源ABA处理结果表明,与WT植株相比,taaa15 - 1a过表达增加了萌发期和萌发后对ABA的敏感性。这些结果表明,taaa15 - 1a通过调控aba相关基因,提高抗氧化胁迫能力,在植物抗旱性中发挥积极作用,在转基因作物中具有潜在的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信