Hydrogen Peroxide Vapor Decontamination of Hazard Group 3 Bacteria and Viruses in a Biosafety Level 3 Laboratory.

IF 0.5 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Applied Biosafety Pub Date : 2022-03-01 Epub Date: 2022-03-15 DOI:10.1089/apb.2021.0022

Charlotte Falaise, Cécile Bouvattier, Guilhem Larigauderie, Valérie Lafontaine, Laurent Berchebru, Audrey Marangon, Valérie Vaude-Lauthier, Françoise Raynaud, Laurent Taysse

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引用次数: 0

Abstract

Aim: This study aimed to validate the efficacy of hydrogen peroxide vapor (HPV) decontamination technology set up in a biosafety level 3 (BSL-3) laboratory on surrogates and hazard group 3 (HG3) agents.

Methods and results: The HPV decontamination system (Bioquell) was assessed with both qualitative and quantitative methods on (1) spore surrogates (Geobacillus stearothermophilus, Bacillus atrophaeus, and Bacillus thuringiensis) in the BSL-3 laboratory and in the material airlock and on (2) HG3 agents (Bacillus anthracis; SARS-CoV-2, Venezuelan equine encephalitis virus [VEE], and Vaccinia virus) in the BSL-3 laboratory. Other HG3 bacteria likely to be handled in the BSL-3 laboratory (Yersinia pestis, Burkholderia mallei, Brucella melitensis, and Francisella tularensis) were excluded from the HPV decontamination assays as preliminary viability tests demonstrated the total inactivation of these agents after 48 h drying on different materials.

Conclusions: The efficacy of HPV decontamination was validated with a reduction in viability of 5-7 log₁₀ for the spores (surrogates and B. anthracis), and for the enveloped RNA viruses. Vaccinia showed a higher resistance to the decontamination process, being dependent on the biological indicator location in the BSL-3 laboratory.

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过氧化氢蒸汽净化生物安全三级实验室中的危险组 3 细菌和病毒。

目的：本研究旨在验证过氧化氢蒸汽（HPV）净化技术在生物安全三级（BSL-3）实验室中对代用品和危险组 3（HG3）制剂的功效：采用定性和定量方法对 HPV 净化系统（Bioquell）进行了评估：(1) BSL-3 实验室和材料气闸中的孢子代用品（嗜热地芽孢杆菌、萎缩性芽孢杆菌和苏云金芽孢杆菌）；(2) BSL-3 实验室中的 HG3 物剂（炭疽杆菌、SARS-CoV-2、委内瑞拉马脑炎病毒 [VEE] 和 Vaccinia 病毒）。其他可能在 BSL-3 实验室中处理的 HG3 细菌（鼠疫耶尔森氏菌、马来伯克霍尔德氏菌、布鲁氏菌和土拉弗氏菌）被排除在 HPV 去污试验之外，因为初步的活力测试表明，这些病原体在不同材料上干燥 48 小时后会完全失活：人类乳头瘤病毒净化的效果得到了验证，孢子（代用品和炭疽杆菌）和包膜 RNA 病毒的存活率降低了 5-7 log10。Vaccinia 对净化过程的抵抗力较强，这取决于 BSL-3 实验室中的生物指标位置。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Biosafety Environmental Science-Management, Monitoring, Policy and Law

CiteScore

2.50

自引率

13.30%

发文量

期刊介绍： Applied Biosafety (APB), sponsored by ABSA International, is a peer-reviewed, scientific journal committed to promoting global biosafety awareness and best practices to prevent occupational exposures and adverse environmental impacts related to biohazardous releases. APB provides a forum for exchanging sound biosafety and biosecurity initiatives by publishing original articles, review articles, letters to the editors, commentaries, and brief reviews. APB informs scientists, safety professionals, policymakers, engineers, architects, and governmental organizations. The journal is committed to publishing on topics significant in well-resourced countries as well as information relevant to underserved regions, engaging and cultivating the development of biosafety professionals globally.