Characterization of Canine Adenovirus Type 2 Virus Infection Pattern in Canine and Human Cell Lines.

IF 1.1 Q4 VIROLOGY

Advances in Virology Pub Date : 2022-01-01 DOI:10.1155/2022/3658970

Madison D Hogans, Will P Kretzschmar, Theresa A Higgins, Atonu Chakrabortty, Rebecca L Nance, Bruce F Smith, Deepa Bedi, Maninder Sandey, Payal Agarwal

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Abstract

Canine adenovirus type 2 (CAV2) is a nonhuman adenovirus with a known ability to infect human and canine cells. The cell surface receptors involved in CAV2 transduction are still unknown. Identification of these would provide valuable information to develop enhanced gene delivery tools and better understand CAV2 biology. CAV2 is erroneously grouped with Ad5 based on the knowledge that CAV2 may transduce using CAR. Therefore, we have evaluated CAV2 and Ad5 (CAV2GFP, Ad5G/L) infection patterns in various canine and human cell lines to determine their different tropisms. Our research demonstrates that CAV2 can successfully infect cells that Ad5 does not infect, and CAV2 infections do not correlate with CAR expression. CAV2 can infect cells that have a low or minimal expression of CAR. Our data suggest that CAV2 transduction is not dependent on the CAR receptor, and thus, it is crucial to find novel CAV2 receptors.

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犬腺病毒2型病毒在犬和人细胞系感染模式的研究。

犬腺病毒2型(CAV2)是一种非人类腺病毒，已知能够感染人类和犬细胞。参与CAV2转导的细胞表面受体尚不清楚。识别这些将为开发增强的基因传递工具和更好地了解CAV2生物学提供有价值的信息。基于CAV2可以使用CAR转导的知识，CAV2被错误地归为Ad5。因此，我们评估了CAV2和Ad5 (CAV2GFP, Ad5G/L)在各种犬和人细胞系中的感染模式，以确定其不同的趋向性。我们的研究表明，CAV2可以成功感染Ad5无法感染的细胞，并且CAV2感染与CAR表达无关。CAV2可以感染CAR低表达或极少表达的细胞。我们的数据表明，CAV2的转导不依赖于CAR受体，因此，寻找新的CAV2受体至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advances in Virology VIROLOGY-

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

22 weeks