Current enzyme-mediated insecticide resistance status of Aedes aegypti populations from a dengue-endemic city in southern Mexico.

IF 2.4 4区医学 Q2 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Salud Publica De Mexico Pub Date : 2023-01-02 DOI:10.21149/13910

Francisco Solís-Santoyo, Américo D Rodríguez, William Black, Karla Saavedra-Rodríguez, Daniel Sánchez-Guillén, Alfredo Castillo-Vera, Rebeca González-Gómez, Alma Delia López-Solis, Rosa Patricia Penilla-Navarro

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引用次数: 1

Abstract

Objective: To identify the enzyme-mediated insecticide resistance in Aedes aegypti in Tapachula, Mexico.

Materials and methods: Biochemical assays were undertaken to determine the enzyme levels in mosquitoes from 22 sites collected in 2018 and 2020 in Tapachula. Results of 2018 were correlated with the resistance to insecticides pub-lished.

Results: Mosquitoes had higher levels than those of the susceptible strain in 2018 and 2020 respectively of α-esterases in 15 and 12 sites; β-esterases in 7 and 6 sites; glutathione-S-transferases in 11 and 19 sites; ρNPA-esterases in 21 and 17 sites; and cytochromes P450 in 20 and 22 sites. In mosquitoes of 2018, there was a moderate correlation between previously documented Malathion resistance ratios and the insensitive acetylcholinesterase (r=0.459, p= 0.03).

Conclusions: The elevated enzyme levels found indicate its contribution to the resistance to pyrethroids and organo-phosphates already published in mosquitoes from Tapachula. Bioassays using enzyme inhibitors resulted in greater mor-tality, confirming that metabolism contributes to resistance.

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墨西哥南部某登革热流行城市埃及伊蚊种群酶介导的杀虫剂抗性现状

目的:鉴定墨西哥塔帕丘拉地区埃及伊蚊酶媒抗药性。材料与方法:采用生化方法对2018年和2020年在塔帕丘拉采集的22个地点的蚊虫进行酶水平测定。2018年的结果与已发表的杀虫剂抗性相关。结果:2018年和2020年蚊虫α-酯酶水平分别高于易感品系15个和12个位点;7和6个位点有β-酯酶;11和19个位点的谷胱甘肽- s转移酶;21和17个位点的ρ npa -酯酶;和细胞色素P450在20和22个位点。在2018年的蚊子中，先前记录的马拉硫磷抗性比率与不敏感的乙酰胆碱酯酶之间存在中度相关性(r=0.459, p= 0.03)。结论:塔帕丘拉蚊对拟除虫菊酯类杀虫剂和有机磷杀虫剂产生抗药性的原因是酶水平升高。使用酶抑制剂的生物测定导致更高的死亡率，证实代谢有助于抵抗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Salud Publica De Mexico PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-

CiteScore

3.30

自引率

21.70%

发文量

审稿时长

34 weeks

期刊介绍： Salud Pública de México se crea en 1959 y comienza a publicarse bimestralmente a partir de 1961; en 1988 inicia una nueva época en la que se refuerza su carácter de publicación científica con evaluación por pares. Es una revista publicada por el Instituto Nacional de Salud Pública (INSP), organismo descentralizado de la Secretaría de Salud de México, dedicado a la investigación, docencia y difusión del conocimiento en salud pública. El INSP, de acuerdo con la normatividad internacional, otorga a la revista independencia editorial.