Investigation of NDM-1 and OXA-48 producing carbapenem resistant Klebsiella pneumoniae ST15 in Iran.

IF 1.3 4区医学 Q4 IMMUNOLOGY

Acta microbiologica et immunologica Hungarica Pub Date : 2023-03-02 DOI:10.1556/030.2023.01945

Hedayat Bozorgi Mohajer, Himen Salimizand, Dahieh Gharanizadeh, Afra Hossainpanahi, Rashid Ramazanzadeh

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Abstract

The aim of this study was to determine the frequency of carbapenem resistant Klebsiella pneumoniae (CRKP) sequence types (STs) in Iran. Samples were collected from three university hospitals in Sanandaj, Iran, from December 2016 to March 2018. Antibiotic susceptibility testing, phenotypic and genotypic detection of carbapenemases were performed. Common K. pneumoniae capsular types were sought for all isolates. The genetic relatedness of isolates was investigated by multilocus sequence typing (MLST). Plasmids were detected by PCR-based Replicon Typing (PBRT). During the study, 67 K. pneumoniae isolates were identified. Of which, 18 (26.9%) isolates were detected as carbapenem-resistant. The most effective antibacterial agent was tigecycline (97%, 65 isolates) followed by imipenem and ertapenem (73.13%, 49 isolates). PCR showed that 13 isolates (19.4%) had blaNDM-1 gene and 5 (7.5%) harbored blaOXA-48. Examination of common capsular types showed that 2 isolates had K2 and 2 others had K54. REP-PCR revealed 10 clones and 11 singleton strains. MLST analysis of CRKP found ST15 as the most common type (13 isolates, 72.2%), but other STs were also detected namely, ST19, ST117, ST1390, and ST1594. ColE1 and IncL/M plasmids were the carriers of blaNDM-1 and blaOXA-48, respectively. The results showed that CRKP spread in our health centers. Our results, therefore, indicate a worrying trend of resistance to carbapenems in K. pneumoniae.

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伊朗产耐碳青霉烯类肺炎克雷伯菌ST15的NDM-1和OXA-48研究

本研究的目的是确定伊朗耐碳青霉烯类肺炎克雷伯菌(CRKP)序列型(STs)的频率。样本于2016年12月至2018年3月从伊朗萨南达杰的三所大学医院采集。进行抗生素药敏试验、碳青霉烯酶表型和基因型检测。所有分离株均寻找常见的肺炎克雷伯菌荚膜类型。采用多位点序列分型(MLST)对分离株进行遗传亲缘性分析。质粒采用基于pcr的复制子分型(PBRT)检测。在研究中鉴定出67株肺炎克雷伯菌。其中18株(26.9%)耐碳青霉烯。最有效的抗菌药物是替加环素(97%，65株)，其次是亚胺培南和厄他培南(73.13%，49株)。PCR结果显示，blaNDM-1基因携带者13株(19.4%)，blaOXA-48基因携带者5株(7.5%)。对常见荚膜类型的检测显示，2株菌株含有K2，另外2株菌株含有K54。REP-PCR检测出10株克隆和11株单株菌株。CRKP的MLST分析发现ST15是最常见的类型(13株，占72.2%)，但也检测到其他STs，即ST19、ST117、ST1390和ST1594。ColE1和IncL/M质粒分别是blaNDM-1和blaOXA-48的载体。结果表明，CRKP在我们的卫生中心传播。因此，我们的结果表明肺炎克雷伯菌对碳青霉烯类的耐药性趋势令人担忧。

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来源期刊

Acta microbiologica et immunologica Hungarica IMMUNOLOGY-MICROBIOLOGY

CiteScore

2.30

自引率

13.30%

发文量

审稿时长

>12 weeks

期刊介绍： AMIH is devoted to the publication of research in all fields of medical microbiology (bacteriology, virology, parasitology, mycology); immunology of infectious diseases and study of the microbiome related to human diseases.