[Quantitative assessment of breast cancer resistance protein during pregnancy in rabbits].

Q3 Biochemistry, Genetics and Molecular Biology
N M Popova, A A Slepnev, Yu V Abalenikhina, A V Shchulkin, E D Rokunov, E N Yakusheva
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引用次数: 0

Abstract

Breast cancer resistance protein (BCRP,ABCG2) is an efflux transporter protein that transports various substrates from the cell to the extracellular space or organ cavities. The aim of this study was a complex assessment of the amount of BCRP during pregnancy in rabbits. The amount of BCRP in samples of the rabbit jejunum, liver, kidney, cerebral cortex, and placenta was determined by enzyme immunoassay, and in human hepatocellular carcinoma (HepG2) cells by the Western blot. To study the mechanisms involved in control of the dynamic BCRP levels during pregnancy, serum concentrations of sex hormones were investigated by radioimmunoassay and relative amounts of constitutive androstane receptor (CAR) and pregnane X receptor (PXR) in these organs were evaluated using the Western blot method. The putative role of CAR and PXR in regulation of the BCRP level by progesterone was evaluated in vitro experiments on HepG2 cells. It was found that amount of BCRP in the jejunum of pregnant rabbits was higher than in the placenta, liver, kidneys, and cerebral cortex. An increase in the amount of BCRP in the liver of rabbits was noted on the 21st day of pregnancy and a tendency to the increase was also detected on the 28th day; in the kidney and cerebral cortex increased BCRP levels were detected on the 28th day and 14th day of pregnancy, respectively, as compared with non-pregnant females. In vitro experiments with HepG2 cells have shown that the increase in the BCRP level is determined by the activating effect of progesterone on PXR.

[家兔妊娠期乳腺癌抵抗蛋白的定量评估]。
乳腺癌耐药蛋白(BCRP,ABCG2)是一种外排转运蛋白,可将各种底物从细胞转运到细胞外空间或器官腔。本研究的目的是对兔怀孕期间BCRP的含量进行复杂的评估。采用酶免疫法检测兔空肠、肝、肾、大脑皮层和胎盘样品中BCRP的含量,采用Western blot法检测人肝癌(HepG2)细胞中BCRP的含量。为了研究妊娠期动态BCRP水平控制的机制,采用放射免疫法检测了血清性激素浓度,并采用Western blot法检测了这些器官中组成型雄甾受体(CAR)和妊娠X受体(PXR)的相对含量。在HepG2细胞的体外实验中,对CAR和PXR在黄体酮调节BCRP水平中的作用进行了评估。结果发现,妊娠兔空肠中BCRP含量高于胎盘、肝脏、肾脏和大脑皮层。妊娠第21天,家兔肝脏BCRP水平升高,第28天有升高趋势;与未怀孕的女性相比,在妊娠第28天和第14天分别检测到肾脏和大脑皮层BCRP水平升高。HepG2细胞体外实验表明,BCRP水平的升高是由黄体酮对PXR的激活作用决定的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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