Nanoencapsulation of R-phycoerytrin extracted from Solieria filiformis improves protein stability and enables its biological application as a fluorescent dye.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2023-01-01 DOI:10.1080/02652048.2023.2168081

Jéssica Roberta Pereira Martins, Antonia Livânia Linhares de Aguiar, Karina Alexandre Barros Nogueira, Acrisio José Uchôa Bastos Filho, Thais da Silva Moreira, Márjory Lima Holanda Araújo, Claudia Pessoa, Josimar O Eloy, Ivanildo José da Silva Junior, Raquel Petrilli

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引用次数: 2

Abstract

We aimed to encapsulate R-PE to improve its stability for use as a fluorescent probe for cancer cells. Purified R-PE from the algae Solieria filiformis was encapsulated in polymeric nanoparticles using PCL. Nanoparticles were characterised and R-PE release was evaluated. Also, cellular uptake using breast and prostate cancer cells were performed. Nanoparticles presented nanometric particle size (198.8 ± 0.06 nm) with low polydispersity (0.13 ± 0.022), negative zeta potential (-18.7 ± 1.10 mV), and 50.0 ± 7.3% encapsulation. FTIR revealed that R-PE is molecularly dispersed in PCL. DSC peak at 307 °C indicates the presence of R-PE in the nanoparticle. Also, in vitro, it was demonstrated low release for nanoparticles and degradation for the free R-PE. Finally, cellular uptake demonstrated the potential of R-PE/PCL nanoparticles for cancer cell detection. Nanoparticles loaded with R-PE can overcome instability and allow application as a fluorescent probe for cancer cells.

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从丝状梭菌中提取的r -植红蛋白的纳米胶囊化提高了蛋白质的稳定性，使其作为荧光染料的生物学应用成为可能。

我们的目的是封装R-PE，以提高其作为癌细胞荧光探针的稳定性。从丝状水藻中纯化的R-PE用PCL包封在聚合物纳米颗粒中。对纳米颗粒进行表征，并评价其R-PE释放度。此外，使用乳腺癌和前列腺癌细胞进行细胞摄取。纳米颗粒粒径为纳米级(198.8±0.06 nm)，多分散性低(0.13±0.022)，zeta电位为负(-18.7±1.10 mV)，包封率为50.0±7.3%。FTIR显示R-PE分子分散在PCL中。307℃时的DSC峰表明纳米颗粒中存在R-PE。此外，在体外，它对纳米颗粒具有低释放性，对游离R-PE具有降解性。最后，细胞摄取证明了R-PE/PCL纳米颗粒用于癌细胞检测的潜力。携带R-PE的纳米颗粒可以克服不稳定性，并允许作为癌细胞的荧光探针应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.