Association of S19W polymorphism in APOA5 gene and serum lipid levels in patients with type 2 diabetic nephropathy.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-03-02 eCollection Date: 2023-09-01 DOI:10.1515/hmbci-2022-0056

Shivasadat Mirabedini, Hadis Musavi, Atieh Makhlough, Mohammad-Bagher Hashemi-Sooteh, Mehryar Zargari

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引用次数: 0

Abstract

Objectives: Type 2 diabetic Mellitus (T2DM) is the most common systemic and endocrine disease in humans, and diabetic nephropathy is one of the most serious complications of this disorder. The polymorphisms in the apolipoprotein A5 (ApoA5) gene are strongly related to hypertriglyceridemia and are considered a predisposing factor for diabetic nephropathy. The current study proposed to examine the association of APOA5-S19W polymorphism with serum lipids levels in patients with type 2 diabetic nephropathy in Mazandaran province.

Methods: This case-control study was designed to determine the association of APOA5-S19W polymorphism with plasma lipid profile in 161 T2DM patients with nephropathy (DN+), without nephropathy (DN-), and in 58 healthy individuals. Lipid profile values were measured using Pars Azmoun commercial kits. S19W variant, one of the polymorphisms of the APOA5 gene, was determined by PCR-restriction fragment length polymorphism (PCR-RFLP) and Taq1 restriction enzyme.

Results: In comparison between the three groups, DN+ had a higher mean TG than DN- and the control group (p<0.001). The incidence of the G allele in DN+ was not significant compared to groups of DN-. Comparing the relationship between the mean of biochemical variables with CC and CG genotypes showed that the mean level of TG in people with CC genotype was increased compared to people with CG genotype in diabetic patients. However, this increase was not significant (p=0.19).

Conclusions: There was no association between SNP APOA5 S19W and serum lipids in diabetic patients with and without nephropathy.

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APOA5基因S19W多态性与2型糖尿病肾病患者血脂水平的相关性。

目的：2型糖尿病（T2DM）是人类最常见的系统性和内分泌疾病，糖尿病肾病是该疾病最严重的并发症之一。载脂蛋白A5（ApoA5）基因的多态性与高甘油三酯血症密切相关，并被认为是糖尿病肾病的诱发因素。本研究旨在检测马赞德兰省2型糖尿病肾病患者APOA5-S19W多态性与血脂水平的关系。方法：本病例对照研究旨在确定161例有肾病（DN+）、无肾病（DN-）的T2DM患者和58名健康人的APOA5-S19W多态性与血脂谱的关系。使用Pars-Azmoun商业试剂盒测量脂质分布值。应用聚合酶链式反应限制性片段长度多态性（PCR-RFLP）和Taq1限制性内切酶对APOA5基因的多态性之一S19W变异体进行了检测。结果：三组比较，DN+组的平均TG高于DN-组和对照组（P结论：糖尿病肾病患者和非肾病患者的SNP APOA5 S19W与血脂无相关性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.