The expression of long non-coding RNA LINC01389, LINC00365, RP11-138J23.1, and RP11-354K4.2 in gastric cancer and their impacts on EMT

IF 2.3 3区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS
Negin Taghehchian , Moein Farshchian , Reihaneh Alsadat Mahmoudian , Ahmad Asoodeh , Mohammad Reza Abbaszadegan
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引用次数: 0

Abstract

Background

Epithelial cancers acquire the epithelial to mesenchymal transition (EMT), which leads tumor cells to invade and metastasize to adjacent and distant tissues. The mechanisms involved in EMT phenotype are controlled by numerous markers as well as signalling pathways. Recently, long non-coding RNAs (lncRNAs) were introduced that play the regulatory role in EMT via crosstalk with EMT-related transcription factors and signalling pathways. The present study aimed to investigate the expression of four lncRNAs in human GC and elucidate their probable role in EMT procedure and the pathogenesis of gastric cancer (GC).

Methods

The expression profile of lncRNAs (LINC01389, LINC00365, RP11-138J23.1, and RP11-354K4.2) and mRNAs (TWIST1, MMP13, MAML1, CD44s, and SALL4) between eighty-three GC and adjacent non-cancerous tissues were assessed by quantitative real-time PCR.

Results

The significant downregulation of LINC00365 (66.3%) and RP11-354K4.2 (62.7%) were observed in GC samples; while the upregulation of LINC01389, RP11-138J23.1, TWIST1, MMP13, MAML1, CD44s, and SALL4 were found in 67.5%, 45.8%, 56.6%, 44.6%, 59%, 55.4%, and 62.7% tumors samples at the mRNA level, respectively. Dysregulation of these lncRNAs and EMT-related markers was significantly related to each other in a variety of clinicopathological features of patients (P < 0.05), indicating positive correlations between LINC01389, LINC00365, RP11-138J23.1, and RP11-354K4.2 with EMT status in GC.

Conclusion

These EMT-regulating lncRNAs may play a key role in transforming gastric epithelial to mesenchymal phenotype and can be novel therapeutic targets for GC. Our results highlight the importance of discovering new lncRNAs involved in gastric carcinogenesis. Detailed molecular mechanisms of these noncoding-coding markers in GC are urgently required.

长链非编码RNA LINC01389、LINC00365、RP11-138J23.1、RP11-354K4.2在胃癌中的表达及其对EMT的影响
上皮性癌症获得上皮向间质转化(EMT),这导致肿瘤细胞侵入并转移到邻近和远处组织。参与EMT表型的机制由许多标记物以及信号通路控制。最近,长链非编码rna (lncrna)通过与EMT相关转录因子和信号通路的串扰在EMT中发挥调节作用。本研究旨在研究四种lncrna在人胃癌中的表达,并阐明它们在EMT过程和胃癌(GC)发病机制中的可能作用。方法采用实时荧光定量PCR检测83例胃癌及癌旁非癌组织中lncRNAs (LINC01389、LINC00365、RP11-138J23.1、RP11-354K4.2)和mrna (TWIST1、MMP13、MAML1、CD44s、SALL4)的表达谱。结果GC样品中LINC00365(66.3%)和RP11-354K4.2(62.7%)显著下调;而LINC01389、RP11-138J23.1、TWIST1、MMP13、MAML1、CD44s和SALL4在mRNA水平上的上调分别在67.5%、45.8%、56.6%、44.6%、59%、55.4%和62.7%的肿瘤样本中出现。这些lncrna和emt相关标志物的失调在患者的多种临床病理特征中具有显著相关性(P <0.05),说明LINC01389、LINC00365、RP11-138J23.1、RP11-354K4.2与GC中EMT状态呈正相关。结论这些调节emt的lncrna可能在胃上皮细胞向间质表型转化过程中发挥关键作用,可能成为胃癌新的治疗靶点。我们的研究结果强调了发现新的lncrna参与胃癌发生的重要性。这些非编码标记物在GC中的详细的分子机制是迫切需要的。
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来源期刊
Molecular and Cellular Probes
Molecular and Cellular Probes 生物-生化研究方法
CiteScore
6.80
自引率
0.00%
发文量
52
审稿时长
16 days
期刊介绍: MCP - Advancing biology through–omics and bioinformatic technologies wants to capture outcomes from the current revolution in molecular technologies and sciences. The journal has broadened its scope and embraces any high quality research papers, reviews and opinions in areas including, but not limited to, molecular biology, cell biology, biochemistry, immunology, physiology, epidemiology, ecology, virology, microbiology, parasitology, genetics, evolutionary biology, genomics (including metagenomics), bioinformatics, proteomics, metabolomics, glycomics, and lipidomics. Submissions with a technology-driven focus on understanding normal biological or disease processes as well as conceptual advances and paradigm shifts are particularly encouraged. The Editors welcome fundamental or applied research areas; pre-submission enquiries about advanced draft manuscripts are welcomed. Top quality research and manuscripts will be fast-tracked.
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