To evaluate the feasibility of cadmium/tellurium (Cd/Te) quantum dots for developing N-terminal Natriuretic Peptide (NT-proBNP) in-vitro diagnostics.

Q2 Health Professions

Journal of immunoassay & immunochemistry Pub Date : 2023-01-02 DOI:10.1080/15321819.2022.2103430

Vani M, Anugya Bhatt, Anoopkumar Thekkuveettil, Sanjay Ganapathy, Jeemon Panniyammakal, Harikrishnan Sivadasanpillai, Manoj Gopi

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引用次数: 1

Abstract

Quantum dots have been widely used for biomedical applications like imaging, targeted drug delivery, and in-vitro diagnostics for better sensitivity. In-vitro diagnostic, lateral flow-based assay systems are gaining attention in the field of biomarker analysis mainly due to ease of test and quick availability of results. In the study, the potential of water-soluble carboxylic (-COOH) functionalized photoluminescent Cadmium Telluride Quantum Dots (CdTe) nanoparticles for lateral flow-based detection of N-terminal Natriuretic Peptide (NT-proBNP) biomarker (for heart failure) detection has been evaluated. Monoclonal antibodies were conjugated with COOH functionalized CdTe with EDC-NHS coupling chemistry, and conjugation was confirmed using FTIR. The CdTe nanoparticle exhibited an emission maximum at 715 nm when it is excited with 375 nm. The COOH functionalized CdTe showed an antigen concentration-dependent linearity in the lateral flow applications when the dye was prepared freshly and used. However, a relative reduction in CdTe quantum dot fluorescence intensity with time was observed. Factors such as low stability could be due to the quenching of the fluorescence of CdTe. This limits its commercial viability as an in-vitro diagnostic tool; thus, modifications of the quantum dots are required to have a stable preparation for its commercial potential for quantifications.

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评价镉碲量子点(Cd/Te)用于n端利钠肽(NT-proBNP)体外诊断的可行性。

量子点已广泛用于生物医学应用，如成像、靶向药物输送和体外诊断，以提高灵敏度。体外诊断，基于横向流动的分析系统在生物标志物分析领域越来越受到关注，主要是因为易于测试和快速获得结果。在这项研究中，研究人员评估了水溶性羧酸(-COOH)功能化光致发光碲化镉量子点(CdTe)纳米颗粒用于侧流检测n端利钠肽(NT-proBNP)生物标志物(用于心力衰竭)的潜力。单克隆抗体通过EDC-NHS偶联化学与COOH功能化的CdTe偶联，并用FTIR证实偶联。当激发波长为375 nm时，CdTe纳米粒子在715 nm处表现出最大发射峰。当染料新鲜制备和使用时，COOH功能化的CdTe在横向流动应用中表现出抗原浓度依赖的线性关系。然而，观察到CdTe量子点荧光强度随时间的相对降低。稳定性低的原因可能是由于CdTe的荧光猝灭。这限制了其作为体外诊断工具的商业可行性;因此，需要对量子点进行修饰，以使其具有稳定的量化商业潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of immunoassay & immunochemistry 医学-免疫学

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Immunoassay & Immunochemistry is an international forum for rapid dissemination of research results and methodologies dealing with all aspects of immunoassay and immunochemistry, as well as selected aspects of immunology. They include receptor assay, enzyme-linked immunosorbent assay (ELISA) in all of its embodiments, ligand-based assays, biological markers of ligand-receptor interaction, in vivo and in vitro diagnostic reagents and techniques, diagnosis of AIDS, point-of-care testing, clinical immunology, antibody isolation and purification, and others.