Antibacterial Efficacy of Walnut Green Husk (WGH) Extract with Zinc Oxide Nanoparticles on Streptococcus Mutans.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Taraneh Movahhed, Maryam Mehrabkhani, Mohsen Arefnezhad, Shokouh Sadat Hamedi, Reza Zare Mahmoudabadi, Fariba Ghanbari, Mahjubeh Rostami
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引用次数: 1

Abstract

Background: Chemical agents, such as Chlorhexidine are used as one of dental plaque control strategy. Researchers are looking for a natural and economic substitute with same antibacterial efficacy and less complications. The aim of this study was to evaluate the antimicrobial efficacy of the Khorasan Razavi walnut green husk (WGH) extract with and without adding ZnO nanoparticles (nZnO) on Streptococcus mutans (S. mutans).

Methods: In this in vitro study, antimicrobial effect of the Hydro-ethanolic extract of WGH, was evaluated against S. mutans. Broth Dilution and Agar diffusion methods were used with 90 tubes containing different dilutions of WGH extract (100 to 0.006 mg/ml). ZnO nanoparticles (nZnO) were added to 45 tubes. Streptococcus mutans was exposed to 15 different serial concentrations of study extracts, from 100 mg/ml to 0.006 mg/ml. Minimum inhibitory concentration (MIC) of the study extracts were determined and zone of inhibition diameter was compared to positive controls (chlorhexidine 0.2%, nZnO), and negative control (sterile distilled water). The differences between the mean diameters, were analyzed by independent sample T- teS.

Results: Minimum inhibitory concentration (MIC) of study extract was found to be 50mg/mL, with adding nZnO, MIC was reduced to 3.12mg/mL. Mean diameter of inhibition zone at 3.12 mg/ml with and without adding ZnO nanoparticles were 17.67±0.57 mm and 8±0.001 mm, respectively, (p-value< 0.001).

Discussion: Adding nZnO could be enhanced antimicrobial efficacy of the WGH extract against S. mutants, while it was still less effective than chlorhexidine.

氧化锌纳米颗粒核桃绿壳提取物对变形链球菌的抗菌作用。
背景:氯己定等化学药物是控制牙菌斑的一种方法。研究人员正在寻找一种具有相同抗菌效果和更少并发症的天然和经济替代品。本研究旨在评价加氧化锌纳米粒(nZnO)和不加氧化锌纳米粒(nZnO)的呼罗珊核桃绿皮(WGH)提取物对变形链球菌(S. mutans)的抑菌效果。方法:采用体外实验的方法,研究了白藜芦醇提取物对变形链球菌的抑菌作用。采用肉汤稀释法和琼脂扩散法,用90根试管装不同稀释度(100 ~ 0.006 mg/ml)的WGH提取物。将氧化锌纳米粒子(nZnO)添加到45根管中。将变形链球菌暴露于15种不同浓度的研究提取物中,从100 mg/ml到0.006 mg/ml。测定各提取物的最低抑菌浓度(MIC),并与阳性对照(0.2%氯己定,nZnO)和阴性对照(无菌蒸馏水)的抑菌区直径进行比较。用独立样本T- teS分析了平均直径之间的差异。结果:最低抑菌浓度(MIC)为50mg/mL,加入nZnO后MIC降至3.12mg/mL。在3.12 mg/ml时,添加ZnO纳米粒子和未添加ZnO纳米粒子的抑制区平均直径分别为17.67±0.57 mm和8±0.001 mm (p值< 0.001)。讨论:添加nZnO可增强白芍提取物对突变链球菌的抑菌效果,但其抑菌效果仍不如氯己定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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