Myoblast proliferation during flight muscle development in Manduca sexta is unaffected by reduced neural signaling

IF 1.7 3区 农林科学 Q2 ENTOMOLOGY
J. Clark Ludwig, Barry Trimmer
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引用次数: 0

Abstract

In holometabolous insects, metamorphosis involves restructuring the musculature to accommodate adult-specific anatomy and behaviors. Evidence from experiments on remodeled muscles, as well as those that develop de novo, suggests that signals from the nervous system support adult muscle development by controlling myoblast proliferation rate. However, the dorsolongitudinal flight muscles (DLMs) of Manduca sexta undergo a mixed developmental program involving larval muscle fibers, and it is not known if neurons play the same role in the formation of these muscles. To address this question, we have blocked the most promising candidate pathways for neural input and examined the DLMs for changes in proliferation. Our results show that DLM development does not depend on neural activity, Hedgehog signaling, or EGF signaling. It remains to be determined how DLM growth is controlled and why neurally mediated proliferation differs between individual muscles.

在飞行肌发育过程中成肌细胞增殖不受神经信号减少的影响
在全变态昆虫中,变态包括重组肌肉组织,以适应成虫特定的解剖结构和行为。来自重塑肌肉和新生肌肉实验的证据表明,来自神经系统的信号通过控制成肌细胞增殖率来支持成年肌肉的发育。然而,六齿Manduca的背纵飞行肌(DLM)经历了一个涉及幼虫肌肉纤维的混合发育程序,目前尚不清楚神经元是否在这些肌肉的形成中发挥着相同的作用。为了解决这个问题,我们阻断了最有希望的神经输入候选途径,并检查了DLM的增殖变化。我们的研究结果表明,DLM的发展不依赖于神经活动、Hedgehog信号或EGF信号。DLM的生长是如何控制的,以及为什么神经介导的增殖在单个肌肉之间不同,还有待确定。
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来源期刊
CiteScore
3.50
自引率
10.00%
发文量
54
审稿时长
60 days
期刊介绍: Arthropod Structure & Development is a Journal of Arthropod Structural Biology, Development, and Functional Morphology; it considers manuscripts that deal with micro- and neuroanatomy, development, biomechanics, organogenesis in particular under comparative and evolutionary aspects but not merely taxonomic papers. The aim of the journal is to publish papers in the areas of functional and comparative anatomy and development, with an emphasis on the role of cellular organization in organ function. The journal will also publish papers on organogenisis, embryonic and postembryonic development, and organ or tissue regeneration and repair. Manuscripts dealing with comparative and evolutionary aspects of microanatomy and development are encouraged.
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