Embryonic growth retardation and altered expression of IGF-II is reciprocal induced by phytocompounds during early gestation in mice.

IF 1.8 4区 生物学 Q4 CELL BIOLOGY
Khamhee Wangsa, Krishnakshi Misra, Upasa Gowala, Indira Sarma, Purba Jyoti Saikia, Hirendra Nath Sarma
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引用次数: 1

Abstract

Methanolic crude extract of Scoparia dulcis (CESD) was orally administered to female mice during the early gestation (day 4-day 8) at a dose of 500mg/kg/day. It induces embryo resorption and morphological changes of fetal maternal tissue. Histomorphology was studied by routine hematoxylin eosin stain. In situ immunofluorescence localization of IGF-II using Texas red showed an ordered expression of the growth factor in the maternal decidual cells, trophoblast cells and the embryo. Western blot analysis showed a gradual increase of IGF-II from D4 to D8 of control females. In contrast, the CESD-treated females showed resorption of embryo on D8 with disorganized in situ expression and lowered IGF-II in fetal maternal tissue. The phytocompounds present in the CESD could modulate either the ER or IGF-II receptors causing reduced IGF-II expression in the target tissues which lead to the failure of embryonic growth during periimplantation.

胚胎生长迟缓和IGF-II的表达改变是由植物化合物在小鼠妊娠早期相互诱导的。
在妊娠早期(第4- 8天)给雌性小鼠口服茯苓甲醇粗提物(CESD) 500 mg/kg/天。诱导胚胎吸收和胎母组织形态改变。常规苏木精伊红染色观察组织形态学。用德州红原位免疫荧光定位IGF-II,发现生长因子在母体蜕膜细胞、滋养层细胞和胚胎中有序表达。Western blot分析显示,对照雌性从D4到D8 IGF-II逐渐升高。相比之下,经cesd处理的雌性在D8时出现胚胎吸收,原位表达紊乱,胎儿母体组织中IGF-II含量降低。CESD中存在的植物化合物可以调节ER或IGF-II受体,导致靶组织中IGF-II表达减少,导致胚胎着床期生长失败。
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来源期刊
Growth factors
Growth factors 生物-内分泌学与代谢
CiteScore
2.60
自引率
0.00%
发文量
20
审稿时长
>12 weeks
期刊介绍: Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications
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