Morphological changes in striated muscle fibres caused by components of the Thiel embalming method.

IF 1.2 4区 医学 Q3 ANATOMY & MORPHOLOGY
Folia morphologica Pub Date : 2024-01-01 Epub Date: 2023-02-22 DOI:10.5603/FM.a2023.0010
Dea Aaldijk, Adrian Reusser, Elisabeth Eppler, Eveline Yao, Valentin Djonov, Sebastian Halm
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引用次数: 0

Abstract

Background: Thiel-fixed body donors are highly valued for surgical training courses. The pronounced flexibility of Thiel-fixed tissue has been postulated to be caused by histologically visible fragmentation of striated muscle. The aim of this study was to analyse whether a specific ingredient, pH, decay, or autolysis could cause this fragmentation in order to modulate the Thiel solution to adapt specimen flexibility specifically to the needs of different courses.

Materials and methods: Striated muscle of the mouse was fixed for different time periods in formalin, Thiel solution, and its individual ingredients, and analysed by light microscopy. Further, pH-values of Thiel solution and its ingredients were measured. In addition, unfixed muscle tissue was histologically analysed including Gram staining to investigate a relationship between autolysis, decomposition, and fragmentation.

Results: Muscle fixed with Thiel solution for 3 months was slightly more fragmentated than muscle fixed for 1 day. Fragmentation was more pronounced after 1 year of immersion. Three individual salt ingredients showed slight fragmentation. Decay and autolysis had no effect on fragmentation, which occurred regardless of the pH of all solutions.

Conclusions: Fragmentation of Thiel-fixed muscle is dependent on fixation time and most likely occurs due to salts present in the Thiel solution. Adjustment of the salt composition in the Thiel solution with verification of the influence on the fixation effect, fragmentation and flexibility of the cadavers could be performed in further studies.

蒂尔防腐法的成分导致横纹肌纤维的形态变化。
背景:蒂尔固定供体在外科培训课程中备受重视。据推测,蒂尔固定组织的明显柔韧性是由组织学上可见的横纹肌碎裂造成的。本研究的目的是分析特定成分、pH 值、衰变或自溶是否会导致这种碎裂,从而调节蒂尔溶液,使标本的柔韧性特别适合不同课程的需要:将小鼠的横纹肌在福尔马林、泰尔溶液及其单独成分中进行不同时间段的固定,并用光学显微镜进行分析。此外,还测量了泰尔溶液及其成分的 pH 值。此外,还对未固定的肌肉组织进行了组织学分析,包括革兰氏染色,以研究自溶、分解和碎裂之间的关系:结果:用泰尔溶液固定三个月的肌肉比固定一天的肌肉碎裂程度略高。浸泡一年后,碎裂更为明显。三种单独的盐成分显示出轻微的破碎。衰变和自溶对碎裂没有影响,无论所有溶液的 pH 值如何,碎裂都会发生:结论:泰尔固定肌肉的碎裂与固定时间有关,很可能是泰尔溶液中的盐造成的。在进一步的研究中,可以调整泰尔溶液中的盐成分,并验证其对固定效果、碎裂和尸体灵活性的影响。
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来源期刊
Folia morphologica
Folia morphologica ANATOMY & MORPHOLOGY-
CiteScore
2.40
自引率
0.00%
发文量
218
审稿时长
6-12 weeks
期刊介绍: "Folia Morphologica" is an official journal of the Polish Anatomical Society (a Constituent Member of European Federation for Experimental Morphology - EFEM). It contains original articles and reviews on morphology in the broadest sense (descriptive, experimental, and methodological). Papers dealing with practical application of morphological research to clinical problems may also be considered. Full-length papers as well as short research notes can be submitted. Descriptive papers dealing with non-mammals, cannot be accepted for publication with some exception.
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