Towards understanding the binding affinity of lipid drug carriers to serum albumin

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry and Physics of Lipids Pub Date : 2023-01-01 DOI:10.1016/j.chemphyslip.2022.105271

Katarzyna Dopierała , Marek Weiss , Martyna Krajewska , Justyna Błońska

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引用次数: 3

Abstract

In the past several years there has been a rapid rise in the use of lipid-based drug formulations. In the case of intravenous drug administration the interaction of lipid carrier with serum albumin is crucial for the distribution of the bioactive molecules in the bloodstream and reaching the target tissue. In this work, we have explored the interaction of serum albumin with three-component lipid monolayer build of palmitoyloleoylphosphatidylcholine (POPC), sphingomyelin (SM), and cholesterol (Chol). Using wide range of lipid compositions and various concentrations of serum albumin we identified the factors governing the lipid-protein binding. Our study revealed that albumin can penetrate selectively the monolayers of POPC/SM/Chol depending on the lipid composition in the mixture. Moreover, the interaction of albumin with monolayer can be controlled by the molecular density of the film and the concentration of protein. The adsorbed albumin exists in the film on the top of lipid monolayer. This behavior may lead to the increase of the size and charge of the lipid carrier and affect the drug transport throughout the bloodstream. The results of this work provide essential physicochemical data that can be used for predicting the pharmacokinetic profile of lipid-based formulations.

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探讨脂质药物载体与血清白蛋白的结合亲和力

在过去的几年中，脂质药物制剂的使用迅速增加。在静脉给药的情况下，脂质载体与血清白蛋白的相互作用对于生物活性分子在血液中的分布和到达靶组织至关重要。在这项工作中，我们探讨了血清白蛋白与棕榈酰磷脂酰胆碱(POPC)、鞘磷脂(SM)和胆固醇(Chol)三组分脂质单层构建的相互作用。利用广泛的脂质组成和不同浓度的血清白蛋白，我们确定了控制脂质-蛋白结合的因素。我们的研究表明，白蛋白可以选择性地穿透POPC/SM/Chol的单层，这取决于混合物中的脂质组成。此外，白蛋白与单层膜的相互作用可以通过膜的分子密度和蛋白的浓度来控制。被吸附的白蛋白存在于脂质单层顶部的膜中。这种行为可能导致脂质载体的大小和电荷的增加，并影响药物在整个血液中的运输。这项工作的结果提供了基本的物理化学数据，可用于预测基于脂质制剂的药代动力学特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemistry and Physics of Lipids 生物-生化与分子生物学

CiteScore

7.60

自引率

2.90%

发文量

审稿时长

40 days

期刊介绍： Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.