Synthesis and bioactivity assessment of Coccinia grandis L. extract encapsulated alginate nanoparticles as an antidiabetic drug lead.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2024-01-01 Epub Date: 2024-01-10 DOI:10.1080/02652048.2023.2282964

Nayomi Deshani De Silva, Anoja Priyadarshani Attanayake, Desiree Nedra Karunaratne, Liyanage Dona Ashanti Menuka Arawwawala, Geethi Kaushalya Pamunuwa

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引用次数: 0

Abstract

Aim: This study aimed to prepare, characterise, and evaluate the antidiabetic activity of Coccinia grandis (L.) extracts encapsulated alginate nanoparticles.

Methods: Alginate nanoparticles were prepared using the ionic gelation method and characterised by encapsulation efficiency %w/w, loading capacity %w/w, particle size analysis, zeta potential, Fourier transform infra-red spectroscopy (FTIR), and scanning electron microscopy (SEM). In vitro antidiabetic activity was also evaluated.

Results: Encapsulation efficiency %w/w, loading capacity %w/w, mean diameter, zeta potential of C. grandis encapsulated alginate nanoparticles ranged from 10.51 ± 0.51 to 62.01 ± 1.28%w/w, 0.39 ± 0.04 to 3.12 ± 0.11%w/w, 191.9 ± 76.7 to 298.9 ± 89.6 nm, -21.3 ± 3.3 to -28.4 ± 3.4 mV, respectively. SEM and FTIR confirmed that particles were in nano range with spherical shape and successful encapsulation of plant extracts into an alginate matrix. The antidiabetic potential of aqueous extract of C. grandis encapsulated alginate nanoparticles (AqCG-ANP) exhibited inhibition in α-amylase, α-glucosidase and dipeptidyl peptidase IV enzymes of 60.8%c/c, 19.1%c/c, and 30.3%c/c, respectively, compared to the AqCG.

Conclusion: The AqCG-ANP exerted promising antidiabetic potential as an antidiabetic drug lead.

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抗糖尿病药物铅包封藻酸盐纳米颗粒的合成及生物活性评价。

目的:制备、表征并评价海藻酸盐纳米颗粒包膜大球藻提取物的抗糖尿病活性。方法:采用离子凝胶法制备海藻酸盐纳米颗粒，并通过包封率%w/w、负载容量%w/w、粒径分析、zeta电位、FTIR和SEM对其进行表征。体外抗糖尿病活性也进行了评估。结果:包封率%w/w、负载量%w/w、平均直径、zeta电位范围分别为10.51±0.51 ~ 62.01±1.28%w/w、0.39±0.04 ~ 3.12±0.11%w/w、191.9±76.7 ~ 298.9±89.6 nm、-21.3±3.3 ~ -28.4±3.4 mV。通过扫描电镜(SEM)和红外光谱(FTIR)分析，证实纳米颗粒呈球形，并成功包封成海藻酸盐基质。包封海藻酸盐纳米颗粒(AqCG- anp)对α-淀粉酶、α-葡萄糖苷酶和DPP-IV酶的抑制率分别为60.8%、19.1%和30.3%。结论:AqCG-ANP作为抗糖尿病先导药物具有良好的降糖潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.