A new approach for the treatment of Alzheimer's disease: insulin-quantum dots.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2024-01-01 Epub Date: 2024-01-10 DOI:10.1080/02652048.2023.2282968

Gamze Camlik, Besa Bilakaya, Yildiz Ozsoy, Ismail Tuncer Degim

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引用次数: 0

Abstract

The potential use of insulin supplementation for Alzheimer's Disease (AD) was aimed to investigate and explore CQDs as an alternative delivery system. CQDs were produced by microwave and characterised. Insulin-loaded Ins-CQDs and in-situ Gel-Ins-CQDs were developed. The in vitro release kinetics, penetrations of insulin through excised sheep nasal mucosa were determined. Toxicity of CQDs were calculated on SH-SY5Y cells. The stability and usability of the prepared formulations were assessed. The insulin release from the solution was 70.75% after 3 hours, while it was 37.51% for in-situ Gel-Ins-CQDs. IC50 value was 52 µM. The mean particle diameters of Ins-CQDs and in-situ Gel-Ins-CQDs varied between 8.35 ± 0.19 to 8.75 ± 0.03 nm during a 6-month period. Zeta potentials ranged from -31.51 ± 1.39 to -24.43 ± 0.26 mV, and PDI values were between 9.8 ± 0.01 to 5.3 ± 3.2%(SD, n = 3) for Ins-CQDs and in-situ Gel-Ins-CQDs, respectively.Our results show that Gel-Ins-CQDs represented a controlled release over time and can be used for AD through the nasal route.

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治疗阿尔茨海默病的新方法:胰岛素量子点。

目的:研究胰岛素补充治疗阿尔茨海默病(AD)的潜在用途，探索CQDs作为一种替代给药系统。方法:微波法制备CQDs并对其进行表征。分别开发了胰岛素负载型Ins-CQDs和原位Gel-Ins-CQDs。测定了胰岛素在体外释放动力学和经切除羊鼻黏膜的渗透。计算CQDs对SH-SY5Y细胞的毒性。评价了制剂的稳定性和可用性。结果:3 h后胰岛素释放率为70.75%，而原位Gel-Ins-CQDs的胰岛素释放率为37.51%。IC50值为52µM。在6个月内，Ins-CQDs和原位Gel-Ins-CQDs的平均粒径在8.35±0.19 ~ 8.75±0.03nm之间变化。in- cqds和原位Gel-Ins-CQDs的Zeta电位范围为-31.51±1.39 ~ -24.43±0.26mV, PDI值分别为9.8±0.01 ~ 5.3±3.2%(SD,n = 3)。结论:我们的研究结果表明Gel-Ins-CQDs具有随时间控释的特点，可以通过鼻腔途径用于治疗AD。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.