Expression of CILP-2 and DDR2 and ultrastructural changes in the articular cartilage of patients with knee osteoarthritis undergoing total knee arthroplasty: a pilot morphological study.

IF 1.2 4区 医学 Q3 PATHOLOGY
Taavi Torga, Siim Suutre, Kalle Kisand, Marina Aunapuu, Andres Arend
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引用次数: 1

Abstract

The aim of the study was to correlate the immunohistochemical expression of cartilage intermediate layer protein 2 (CILP-2) and discoidin domain receptor 2 (DDR2), and the ultrastructural changes in the cartilage with the degree of articular cartilage damage in osteoarthritis (OA) patients. Cartilage samples were obtained from twenty patients aged from 46 to 68 years undergoing total knee arthroplasty. In each patient, medial and lateral tibial plateau samples were analysed applying OARSI histopathology grading. Positive correlation was noted between the extent of CILP-2 staining intensity and OARSI grades. Abundant staining for CILP-2 was found in the superficial and middle layers and in the pericellular matrix (PCM) of the deep zone. Transmission electron microscopy studies demonstrated strong damage of chondrocytes, the organelles were often diminished or focally aggregated. As a characteristic finding, PCM was frequently expanded, which may reflect a pathogenic step in OA progression. In conclusion, CILP-2 may potentially be a relevant marker of OA progression as its expression correlated better with cartilage damage than the known marker of articular cartilage damage, DDR2.

Abstract Image

全膝关节置换术后膝关节骨性关节炎患者关节软骨中CILP-2和DDR2的表达及超微结构变化:一项初步形态学研究
本研究旨在探讨骨关节炎(OA)患者软骨中间层蛋白2 (CILP-2)和盘状蛋白结构域受体2 (DDR2)的免疫组化表达及软骨超微结构变化与关节软骨损伤程度的相关性。软骨样本取自20例年龄从46岁到68岁接受全膝关节置换术的患者。在每位患者中,应用OARSI组织病理学分级分析胫骨平台内侧和外侧样本。CILP-2染色强度与OARSI分级呈正相关。在浅层、中间层和深区细胞周基质(PCM)中可见丰富的CILP-2染色。透射电镜研究显示软骨细胞严重损伤,细胞器经常减少或局部聚集。作为一个特征性发现,PCM经常扩大,这可能反映了OA进展的一个致病步骤。综上所述,与已知的关节软骨损伤标志物DDR2相比,CILP-2的表达与软骨损伤的相关性更好,因此CILP-2可能是OA进展的相关标志物。
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来源期刊
Medical Molecular Morphology
Medical Molecular Morphology 医学-病理学
CiteScore
2.90
自引率
5.60%
发文量
30
审稿时长
>12 weeks
期刊介绍: Medical Molecular Morphology is an international forum for researchers in both basic and clinical medicine to present and discuss new research on the structural mechanisms and the processes of health and disease at the molecular level. The structures of molecules, organelles, cells, tissues, and organs determine their normal function. Disease is thus best understood in terms of structural changes in these different levels of biological organization, especially in molecules and molecular interactions as well as the cellular localization of chemical components. Medical Molecular Morphology welcomes articles on basic or clinical research in the fields of cell biology, molecular biology, and medical, veterinary, and dental sciences using techniques for structural research such as electron microscopy, confocal laser scanning microscopy, enzyme histochemistry, immunohistochemistry, radioautography, X-ray microanalysis, and in situ hybridization. Manuscripts submitted for publication must contain a statement to the effect that all human studies have been reviewed by the appropriate ethics committee and have therefore been performed in accordance with the ethical standards laid down in an appropriate version of the 1964 Declaration of Helsinki. It should also be stated clearly in the text that all persons gave their informed consent prior to their inclusion in the study. Details that might disclose the identity of the subjects under study should be omitted.
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