A simple and cost-effective method for studying anoxia tolerance in plants

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2023-01-28 DOI:10.1002/aps3.11509

Orla L. Sherwood, Rebecca Carroll, Stephen Burke, Paul F. McCabe, Joanna Kacprzyk

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Abstract

Premise

We developed a novel, cost-effective protocol that facilitates testing anoxia tolerance in plants without access to specialized equipment.

Methods and Results

Arabidopsis thaliana and barley (Hordeum vulgare) seedlings were treated in airtight 2-L Kilner jars. An anoxic atmosphere was generated using Oxoid AnaeroGen 2.5-L sachets placed on in-house, custom-built wire stands. The performed experiments confirmed a higher sensitivity to low oxygen stress previously observed in anac017 A. thaliana mutants and the positive effect of exogenous sucrose on anoxia tolerance reported by previous studies in A. thaliana. Barley seedlings displayed typical responses to anoxia treatment, including shoot growth cessation and the induction of marker genes for anaerobic metabolism and ethylene biosynthesis in root tissue.

Conclusions

The results validate the novel method as an inexpensive, simple alternative for testing anoxia tolerance in plants, where access to an anaerobic workstation is not possible. The novel protocol requires minimum investment and is easily adaptable.

Abstract Image

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研究植物耐缺氧性的一种简单而经济的方法

我们开发了一种新颖的，具有成本效益的方案，便于在没有专门设备的情况下测试植物的耐氧性。方法与结果拟南芥和大麦(Hordeum vulgare)幼苗在密闭的2-L Kilner罐中处理。在室内定制的金属支架上放置2.5 l的Oxoid AnaeroGen小袋，产生缺氧气氛。实验证实了先前在anac017拟南芥突变体中观察到的对低氧胁迫的更高敏感性，以及先前研究报道的外源蔗糖对拟南芥耐氧性的积极影响。大麦幼苗对缺氧处理表现出典型的反应，包括茎部生长停止以及根组织中厌氧代谢和乙烯生物合成标记基因的诱导。结果验证了这种新方法是一种廉价、简单的替代方法，可用于在无法获得厌氧工作站的植物中测试耐氧性。该协议投资最小，适应性强。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464