Synthesis characterisation and neuroprotectivity of Silybum marianum extract loaded chitosan nanoparticles.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2023-01-01 Epub Date: 2023-01-25 DOI:10.1080/02652048.2023.2167012

Hatice Feyzan Ay, Serap Yesilkir-Baydar, Rabia Cakir-Koc

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引用次数: 0

Abstract

Aim: Silybum marianum extract (SME) possesses neuroprotective potency through its high antioxidant content. We attempted to increase the effectiveness of SME by encapsulating them in chitosan. Neuroprotective potency of SME and SME-loaded chitosan nanoparticles (SME-CNPs) were shown in SH-SY5Y cell line against H₂O₂-induced oxidative stress.

Methods: We produced CNPs and SME-CNPs by ionic gelation method and properly determined their physical characteristics. Encapsulation efficiency, loading capacity, and in vitro release tests were performed for SME-CNPs. The neurotoxicity and neuroprotective efficiency in SH-SY5Y cell line against H₂O₂ was also investigated.

Results: The size of SME-CNPs was 168.2 ± 11.12 nm with zeta potential 10.6 ± 1.0 mV. The encapsulation efficiency and loading capacity were successfully achieved at 96.6% and 1.89% respectively. SME and SME-CNPs improved cell viability higher than 80%, and SME-CNPs exhibited significant neuroprotective effects against H₂O₂ damage.

Conclusions: It was concluded that SME and SME-CNPs highly prevent damage caused by H₂O₂ and reduce cell damage in vitro by their neuroprotective effects.

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水飞蓟提取物负载壳聚糖纳米颗粒的合成特征和神经保护作用

目的：水飞蓟提取物（SME）具有很高的抗氧化剂含量，因而具有保护神经的功效。我们试图通过将 SME 包裹在壳聚糖中来提高其功效。在 SH-SY5Y 细胞系中显示了 SME 和 SME 负载壳聚糖纳米颗粒（SME-CNPs）对 H2O2 诱导的氧化应激的神经保护效力：方法：我们采用离子凝胶法制备了 CNPs 和 SME-CNPs，并正确测定了它们的物理特性。对 SME-CNPs 进行了包封效率、负载能力和体外释放试验。此外，还研究了 SME-CNPs 在 SH-SY5Y 细胞系中对 H2O2 的神经毒性和神经保护作用：结果：SME-CNPs 的尺寸为 168.2 ± 11.12 nm，zeta 电位为 10.6 ± 1.0 mV。封装效率和负载能力分别达到 96.6% 和 1.89%。SME和SME-CNPs对细胞活力的改善超过80%，SME-CNPs对H2O2损伤具有显著的神经保护作用：结论：SME 和 SME-CNPs 可有效防止 H2O2 造成的损伤，并通过其神经保护作用减少体外细胞损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.