Combined toxicity of soil antimony and cadmium on earthworm Eisenia fetida: Accumulation, biomarker responses and joint effect

IF 6.6 Q1 ENGINEERING, ENVIRONMENTAL
Zhinan Xu, Zaifu Yang, Wenjun Shu, Tong Zhu
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引用次数: 17

Abstract

Antimony (Sb) and cadmium (Cd) coexisted in urban contaminated soil due to textile printing and dyeing waste water, but the environmental risks and ecotoxicological effects they caused are poorly understood. In this study, earthworm Eisenia fetida was exposed into soil spiked with Sb, Cd and their mixture, and multiple biomarker responses (protein, malonaldehyde (MDA), metallothionein (MT), reactive oxygen species (ROS), catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and glutathione S-transferasesase (GST)) were measured to evaluate biological health status and joint effect via Biomarker rResponse Index (BRI) and Effect Addition Index (EAI). The results showed that earthworm could not accumulate Sb effectively, but Cd accumulation by earthworm indicated a well dose-response relationship between issue content and treatment level. Secondly, the protein content was overall decreased, and the contents of MDA, MT and ROS as well as the activities of SOD, POD, CAT, GST and ROS were increased, suggesting membrane lipid peroxidation and waken-up antioxidant capacity, among which GST was the most sensitive. Furthermore, severe alterations for health status were always found except under the antimony dosage of 5 mg/kg, and earthworm health status was more sensitive in presence of single Cd. According to EAI, a clear joint effect of antagonism was observed at whole range of combined treatment levels. This is a key study providing biomarker responses of soil Sb and evaluating the joint effect of Sb and Cd at different contents using earthworm Eisenia fetida, BRI and EAI.

土壤锑和镉对蚯蚓的联合毒性:积累、生物标志物反应和联合效应
城市印染废水污染土壤中锑(Sb)和镉(Cd)共存,但其造成的环境风险和生态毒理学效应尚不清楚。本研究将蚯蚓Eisenia fetida暴露于添加Sb、Cd及其混合物的土壤中,通过生物标志物反应指数(BRI)和谷胱甘肽s -转移酶(GST),测定多种生物标志物(蛋白质、丙二醛(MDA)、金属硫蛋白(MT)、活性氧(ROS)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和谷胱甘肽s -转移酶(GST)),评价其生物健康状况和联合效应。结果表明,蚯蚓不能有效地积累锑,但蚯蚓对镉的积累与处理水平呈良好的量效关系。其次,蛋白质含量整体下降,MDA、MT、ROS含量以及SOD、POD、CAT、GST、ROS活性升高,提示膜脂过氧化和觉醒抗氧化能力,其中GST最为敏感。此外,除锑剂量为5 mg/kg外,蚯蚓的健康状况都发生了严重的变化,并且在单一镉的存在下,蚯蚓的健康状况更为敏感。根据EAI,在整个联合处理水平范围内观察到明显的联合拮抗效应。本研究是利用长条蚯蚓(Eisenia fetida)、BRI和EAI研究土壤Sb和Cd在不同含量下的生物标志物响应和联合效应的关键研究。
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来源期刊
Journal of hazardous materials letters
Journal of hazardous materials letters Pollution, Health, Toxicology and Mutagenesis, Environmental Chemistry, Waste Management and Disposal, Environmental Engineering
CiteScore
10.30
自引率
0.00%
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0
审稿时长
20 days
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