Pristane induced lupus mice as a model for neuropsychiatric lupus (NPSLE).

IF 4.7 2区 心理学 Q1 BEHAVIORAL SCIENCES
Yang Yun, Xuejiao Wang, Jingyi Xu, Chenye Jin, Jingyu Chen, Xueru Wang, Jianing Wang, Ling Qin, Pingting Yang
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引用次数: 2

Abstract

Background: The pristane-induced lupus (PIL) model is a useful tool for studying environmental-related systemic lupus erythematosus (SLE). However, neuropsychiatric manifestations in this model have not been investigated in detail. Because neuropsychiatric lupus (NPSLE) is an important complication of SLE, we investigated the neuropsychiatric symptoms in the PIL mouse model to evaluate its suitability for NPSLE studies.

Results: PIL mice showed olfactory dysfunction accompanied by an anxiety- and depression-like phenotype at month 2 or 4 after pristane injection. The levels of cytokines (IL-1β, IFN-α, IFN-β, IL-10, IFN-γ, IL-6, TNF-α and IL-17A) and chemokines (CCL2 and CXCL10) in the brain and blood-brain barrier (BBB) permeability increased significantly from week 2 or month 1, and persisted throughout the observed course of the disease. Notably, IgG deposition in the choroid plexus and lateral ventricle wall were observed at month 1 and both astrocytes and microglia were activated. Persistent activation of astrocytes was detected throughout the observed course of the disease, while microglial activation diminished dramatically at month 4. Lipofuscin deposition, a sign of neuronal damage, was detected in cortical and hippocampal neurons from month 4 to 8.

Conclusion: PIL mice exhibit a series of characteristic behavioral deficits and pathological changes in the brain, and therefore might be suitable for investigating disease pathogenesis and for evaluating potential therapeutic targets for environmental-related NPSLE.

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普里斯坦诱导狼疮小鼠作为神经精神性狼疮(NPSLE)模型。
背景:前列腺素诱导狼疮(PIL)模型是研究环境相关性系统性红斑狼疮(SLE)的有效工具。然而,该模型的神经精神表现尚未得到详细研究。由于神经精神性狼疮(NPSLE)是SLE的重要并发症,我们研究了PIL小鼠模型中的神经精神症状,以评估其对NPSLE研究的适用性。结果:注射普里斯坦后第2个月或第4个月,PIL小鼠出现嗅觉功能障碍并伴有焦虑和抑郁样表型。脑和血脑屏障(BBB)通透性中的细胞因子(IL-1β、IFN-α、IFN-β、IL-10、IFN-γ、IL-6、TNF-α和IL-17A)和趋化因子(CCL2和CXCL10)水平从第2周或第1个月开始显著升高,并在整个病程中持续存在。值得注意的是,在第1个月时,在脉络膜丛和侧脑室壁上观察到IgG沉积,星形胶质细胞和小胶质细胞都被激活。星形胶质细胞的持续激活在整个观察过程中被检测到,而小胶质细胞的激活在第4个月时急剧减少。第4至8个月,皮质和海马神经元中检测到脂褐素沉积,这是神经元损伤的标志。结论:PIL小鼠在大脑中表现出一系列特征性的行为缺陷和病理改变,因此可能适合研究疾病的发病机制和评估环境相关NPSLE的潜在治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Behavioral and Brain Functions
Behavioral and Brain Functions 医学-行为科学
CiteScore
5.90
自引率
0.00%
发文量
11
审稿时长
6-12 weeks
期刊介绍: A well-established journal in the field of behavioral and cognitive neuroscience, Behavioral and Brain Functions welcomes manuscripts which provide insight into the neurobiological mechanisms underlying behavior and brain function, or dysfunction. The journal gives priority to manuscripts that combine both neurobiology and behavior in a non-clinical manner.
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