Immunohistochemistry for IRTA1 and MNDA helps differentiate gastric MALT lymphoma from chronic gastritis/reactive lymphocyte hyperplasia.

IF 0.9 Q4 HEMATOLOGY

Journal of Clinical and Experimental Hematopathology Pub Date : 2022-12-28 DOI:10.3960/jslrt.22021

Yoshiyuki Ayada, Takuro Igawa, Yusuke Naoi, Kyosuke Horikawa, Tetsuya Tabata, Takehiro Tanaka, Tadashi Yoshino

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引用次数: 1

Abstract

It is difficult to histologically differentiate extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma) from chronic gastritis (CG)/ reactive lymphoid hyperplasia (RLH). To determine whether immunohistochemistry for IRTA1 and MNDA can differentiate gastric MALT lymphoma from CG/RLH, we investigated 81 stomach biopsy specimens [Wotherspoon grade (WG) 1, 11 cases; WG 2, 9 cases; WG 3, 20 cases; WG 4, 31 cases; and WG 5, 10 cases]. According to a previously reported algorithm involving PCR for immunoglobulin heavy (IgH) chain locus rearrangement, all 81 cases were divided into three groups: CG/RLH (55 cases), MALT lymphoma (19 cases) groups, and IgH undetectable group (7 cases). We analyzed the CG/RLH and MALT lymphoma groups. The median percentage of IRTA1-positive cells was 0% (range 0%-90.6%) in the CG/RLH group and 43.5% (range 0%-97.6%) in the MALT lymphoma group (p < 0.0001). The median percentage of MNDA-positive cells was 32.4% (range 0%-97.6%) in the CG/RLH group and 55.1% (range 0%-97.6%) in the MALT lymphoma group (p = 0.0044). These results indicate that immunohistochemistry for IRTA1 and MNDA can help differentiate gastric MALT lymphoma from CG/RLH.

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免疫组织化学检测IRTA1和MNDA有助于区分胃MALT淋巴瘤与慢性胃炎/反应性淋巴细胞增生。

从组织学上很难区分结外粘膜相关淋巴组织边缘区淋巴瘤(MALT淋巴瘤)与慢性胃炎(CG)/反应性淋巴样增生(RLH)。为了确定IRTA1和MNDA的免疫组化是否可以区分胃MALT淋巴瘤和CG/RLH，我们研究了81例胃活检标本[Wotherspoon分级(WG) 1,11例;WG 2,9例;WG 3,20例;WG 4,31例;WG 5,10例]。根据先前报道的免疫球蛋白重(IgH)链位点重排PCR算法，将81例患者分为CG/RLH组(55例)、MALT淋巴瘤组(19例)和IgH未检出组(7例)。我们分析了CG/RLH和MALT淋巴瘤组。irta1阳性细胞的中位数百分比在CG/RLH组为0%(范围为0%-90.6%)，在MALT淋巴瘤组为43.5%(范围为0%-97.6%)(p < 0.0001)。在CG/RLH组中，mnda阳性细胞的中位数百分比为32.4%(范围0%-97.6%)，在MALT淋巴瘤组中为55.1%(范围0%-97.6%)(p = 0.0044)。这些结果表明，IRTA1和MNDA的免疫组化可以帮助区分胃MALT淋巴瘤和CG/RLH。

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