In vivo imaging of inflammatory response in cancer research.

IF 5 3区 医学 Q2 IMMUNOLOGY
Yoshinobu Konishi, Kenta Terai
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引用次数: 0

Abstract

Inflammation can contribute to the development and progression of cancer. The inflammatory responses in the tumor microenvironment are shaped by complex sequences of dynamic intercellular cross-talks among diverse types of cells, and recapitulation of these dynamic events in vitro has yet to be achieved. Today, intravital microscopy with two-photon excitation microscopes (2P-IVM) is the mainstay technique for observing intercellular cross-talks in situ, unraveling cellular and molecular mechanisms in the context of their spatiotemporal dynamics. In this review, we summarize the current state of 2P-IVM with fluorescent indicators of signal transduction to reveal the cross-talks between cancer cells and surrounding cells including both immune and non-immune cells. We also discuss the potential application of red-shifted indicators along with optogenetic tools to 2P-IVM. In an era of single-cell transcriptomics and data-driven research, 2P-IVM will remain a key advantage in delivering the missing spatiotemporal context in the field of cancer research.

Abstract Image

Abstract Image

癌症研究中炎症反应的体内成像。
炎症可导致癌症的发生和发展。肿瘤微环境中的炎症反应是由不同类型细胞之间动态的细胞间交叉作用的复杂序列形成的,而这些动态事件在体外的再现尚待实现。如今,使用双光子激发显微镜(2P-IVM)的体外显微镜已成为原位观察细胞间交叉作用的主流技术,可在时空动态的背景下揭示细胞和分子机制。在这篇综述中,我们总结了利用信号转导荧光指示剂揭示癌细胞与周围细胞(包括免疫细胞和非免疫细胞)之间交叉联系的 2P-IVM 现状。我们还讨论了红移指示剂和光遗传学工具在 2P-IVM 中的潜在应用。在单细胞转录组学和数据驱动研究的时代,2P-IVM 仍将是癌症研究领域提供缺失的时空背景的关键优势。
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来源期刊
CiteScore
11.10
自引率
1.20%
发文量
45
审稿时长
11 weeks
期刊介绍: Inflammation and Regeneration is the official journal of the Japanese Society of Inflammation and Regeneration (JSIR). This journal provides an open access forum which covers a wide range of scientific topics in the basic and clinical researches on inflammation and regenerative medicine. It also covers investigations of infectious diseases, including COVID-19 and other emerging infectious diseases, which involve the inflammatory responses. Inflammation and Regeneration publishes papers in the following categories: research article, note, rapid communication, case report, review and clinical drug evaluation.
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