WITHDRAWN: Activation Of Wnt/Β-Catenin Signaling in Epcamhigh/CD44+ Cells Endow Colorectal Cancer With Tumor Proliferation And Oxaliplatin Chemoresistance

IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Fengqiang Zhou, Yanmei Qi, Zhen Geng, Baozhong Ding, Lei Liu
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Abstract

Since the authors are not responding to the editor’s requests to fulfill the editorial requirement, therefore, the article has been withdrawn.

Bentham Science apologizes to the readers of the journal for any inconvenience this may have caused.

The Bentham Editorial Policy on Article Withdrawal can be found at https://benthamscience.com/editorial-policies-main.php

Bentham science disclaimer: It is a condition of publication that manuscripts submitted to this journal have not been published and will not be simultaneously submitted or published elsewhere. Furthermore, any data, illustration, structure or table that has been published elsewhere must be reported, and copyright permission for reproduction must be obtained. Plagiarism is strictly forbidden, and by submitting the article for publication the authors agree that the publishers have the legal right to take appropriate action against the authors, if plagiarism or fabricated information is discovered. By submitting a manuscript the authors agree that the copyright of their article is transferred to the publishers if and when the article is accepted for publication.

EpCAMhigh/CD44+ 细胞中的 Wnt/β-Catenin 信号激活赋予结直肠癌肿瘤增殖和奥沙利铂化疗耐药性。
目的:本研究调查了EpCAMhigh/CD44+癌干细胞在结直肠癌中的确切比例、体外增殖潜力和奥沙利铂化疗耐药性。背景:背景:结直肠癌干细胞(CSC)在肿瘤致病性和化疗耐药性中起着至关重要的作用。多项研究表明,与肿瘤复发和转移相关的JAK/STAT、NOTCH和Wnt/-catenin通路有助于CSCs的增殖和维持。CSCs通过改善DNA损伤修复、改变细胞周期检查点和清除活性氧,从而对化疗放疗产生抗药性,导致患者预后不良:这项研究旨在确定 EpCAMhigh/CD44+ 癌症干细胞在结直肠癌中的精确比例、体外增殖能力和奥沙利铂化疗耐药性水平。研究还对其潜在过程进行了调查:方法:应用荧光激活细胞分选技术(FACS)从三种人类结直肠癌细胞系(HCT116、HT29和LoVo)中分离出EpCAMhigh/CD44+细胞群,并量化了每种细胞系中EpCAMhigh/CD44+细胞的平均比例。通过 CCK8 检测法评估了这些细胞与亲本细胞的增殖能力和对奥沙利铂的化疗耐药性。免疫印迹法检测了激活的信号通路:结果:EpCAMhigh/CD44+亚群约占人类结直肠癌细胞系总数的4.98±1.24%,与亲代细胞相比,EpCAMhigh/CD44+细胞的增殖能力更强,对奥沙利铂的化疗耐药性也更强。在EpCAMhigh/CD44+的HCT116细胞中,wnt/β-catenin信号通路被激活:结论:EpCAMhigh/CD44+细胞中Wnt/β-Catenin信号通路的激活赋予了结直肠癌肿瘤增殖和奥沙利铂化疗耐药性。
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来源期刊
CiteScore
3.10
自引率
5.60%
发文量
327
审稿时长
7.5 months
期刊介绍: Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.
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