Hypoxia-tolerant apical-out intestinal organoids to model host-microbiome interactions.

IF 6.7 1区 工程技术 Q1 CELL & TISSUE ENGINEERING
Panagiota Kakni, Barry Jutten, Daniel Teixeira Oliveira Carvalho, John Penders, Roman Truckenmüller, Pamela Habibovic, Stefan Giselbrecht
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引用次数: 3

Abstract

Microbiome is an integral part of the gut and is essential for its proper function. Imbalances of the microbiota can be devastating and have been linked with several gastrointestinal conditions. Current gastrointestinal models do not fully reflect the in vivo situation. Thus, it is important to establish more advanced in vitro models to study host-microbiome/pathogen interactions. Here, we developed for the first time an apical-out human small intestinal organoid model in hypoxia, where the apical surface is directly accessible and exposed to a hypoxic environment. These organoids mimic the intestinal cell composition, structure and functions and provide easy access to the apical surface. Co-cultures with the anaerobic strains Lactobacillus casei and Bifidobacterium longum showed successful colonization and probiotic benefits on the organoids. These novel hypoxia-tolerant apical-out small intestinal organoids will pave the way for unraveling unknown mechanisms related to host-microbiome interactions and serve as a tool to develop microbiome-related probiotics and therapeutics.

Abstract Image

Abstract Image

Abstract Image

耐缺氧的尖向外肠道类器官模拟宿主-微生物组相互作用。
微生物群是肠道的一个组成部分,对肠道的正常功能至关重要。微生物群的不平衡可能是毁灭性的,并与几种胃肠道疾病有关。目前的胃肠道模型不能完全反映体内情况。因此,建立更先进的体外模型来研究宿主-微生物组/病原体的相互作用是很重要的。在这里,我们首次开发了缺氧条件下的人类小肠类器官模型,其中根尖表面可以直接接触并暴露在缺氧环境中。这些类器官模拟了肠细胞的组成、结构和功能,并提供了进入肠根尖表面的便利。与厌氧菌株干酪乳杆菌和长双歧杆菌共培养显示出在类器官上成功定植和益生菌效益。这些新型耐缺氧的尖向外小肠类器官将为揭示宿主-微生物组相互作用的未知机制铺平道路,并作为开发微生物组相关益生菌和治疗的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Tissue Engineering
Journal of Tissue Engineering Engineering-Biomedical Engineering
CiteScore
11.60
自引率
4.90%
发文量
52
审稿时长
12 weeks
期刊介绍: The Journal of Tissue Engineering (JTE) is a peer-reviewed, open-access journal dedicated to scientific research in the field of tissue engineering and its clinical applications. Our journal encompasses a wide range of interests, from the fundamental aspects of stem cells and progenitor cells, including their expansion to viable numbers, to an in-depth understanding of their differentiation processes. Join us in exploring the latest advancements in tissue engineering and its clinical translation.
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