Protective Effects of Cinnamic Acid Against Hyperglycemia Induced Oxidative Stress and Inflammation in HepG2 Cells.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Reports of Biochemistry and Molecular Biology Pub Date : 2023-04-01 DOI:10.52547/rbmb.12.1.1

Mohammad Yazdi, Amirhossein Nafari, Mojgan Azadpour, Mahdi Alaee, Forouzan Hadipour Moradi, Razieh Choghakhori, Maryam Hormozi, Hassan Ahmadvand

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Abstract

Background: Cinnamic acid, a phenylpropanoid acid, has been investigated as a potential alternative therapy for diabetes and its complications in some studies.

Methods: In the first stage, the viability of HepG2 cells at different concentrations of glucose and CA was assessed by MTT assay. Oxidative stress markers) CAT, GPx, GSH, and MDA) were measured spectrophotometrically. After RNA extraction, the effect of different concentrations of CA on the expression of DPP4 and inflammatory factors (IL-6, NF- κB) in HepG2 cells was assessed using real-time PCR.

Results: In HepG2 cells, CA increased catalase and glutathione peroxidase activity and GSH production in a dose-dependent manner in the presence of high glucose concentrations, with the greatest effect seen at a concentration of 75 mg/ml. Also, it reduced the amount of MDA in high-glucose HepG2 cells. Furthermore, CA decreased the expression of DPP4, NF- κB, and IL-6 genes in HepG2 cells in the presence of high glucose levels.

Conclusions: The results of our study indicated that CA reduced hyperglycemia-induced complications in HepG2 cells by decreasing inflammatory gene expression, including IL-6 and NF- κB and inhibiting the expression of DPP4, and limiting oxidative stress.

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肉桂酸对高血糖诱导的HepG2细胞氧化应激和炎症的保护作用。

背景：肉桂酸是一种苯丙酸，在一些研究中已被研究为糖尿病及其并发症的潜在替代疗法。方法：在第一阶段，用MTT法测定不同浓度葡萄糖和CA对HepG2细胞的活力。氧化应激标记物）CAT、GPx、GSH和MDA）进行分光光度测定。RNA提取后，使用实时PCR评估不同浓度的CA对HepG2细胞中DPP4和炎症因子（IL-6、NF-κB）表达的影响，在75mg/ml的浓度下观察到最大的效果。此外，它还降低了高糖HepG2细胞中MDA的含量。此外，在高糖水平下，CA降低了HepG2细胞中DPP4、NF-κB和IL-6基因的表达。结论：我们的研究结果表明，CA通过降低炎症基因（包括IL-6和NF-κB）的表达、抑制DPP4的表达和限制氧化应激来减少高血糖诱导的HepG2细胞并发症。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.80

自引率

23.50%

发文量

审稿时长

10 weeks

期刊介绍： The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.