Expression Level of lncRNA CYTOR in Iranian Cervical Cancer Patients.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Reports of Biochemistry and Molecular Biology Pub Date : 2023-04-01 DOI:10.52547/rbmb.12.1.120

Ali Rajabi, Narges Dastmalchi, Neda Shokri, Samaneh Tayefeh-Gholami, Seyyed Mohammad Yaghoubi, Reza Safaralizadeh

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引用次数: 0

Abstract

Background: A critical role has been known for lncRNAs in the initiation and development of cancers. Therefore, lncRNAs have been reported as the possible biomarkers in relation to the diagnosis and therapy of malignancies. This project examined the change in CYTOR lncRNA expression in human cervical cancer samples as compared with adjacent healthy ones.

Methods: We provided one hundred fifteen pairs of tumorous and adjacent healthy tissue specimens of cervical cancer patients. RNAs were isolated from tissue specimens and cDNAs were synthesized. We considered quantitative Real-time PCR (qRT-PCR) to examine the expression levels of CYTOR lncRNA. In addition, the biomarker activity of CYTOR and the associations between the lncRNA and clinicopathological characteristics were evaluated.

Results: The significant increased expression of CYTOR was obtained in cancerous samples as compared with non-cancerous ones (P< 0.0001). A significant correlation was indicated between CYTOR expression and the squamous subtype of cervical cancer (p=0.046). The receiver operating characteristic (ROC) curve-related AUC (area under the curve), specificity, and sensitivity were calculated 0.88, 81.74%, and 80%, respectively, which may introduce CYTOR as a potential biomarker.

Conclusion: CYTOR may be an effective oncogene and biomarker in cervical cancer cases given its increased expression in human cervical cancer tissues.

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伊朗子宫颈癌症患者lncRNA细胞因子的表达水平。

背景：已知lncRNA在癌症的发生和发展中起着关键作用。因此，lncRNA已被报道为与恶性肿瘤诊断和治疗相关的可能生物标志物。该项目检测了与邻近健康人相比，人类癌症样本中细胞因子lncRNA表达的变化。方法：我们提供了115对癌症患者的肿瘤和癌旁健康组织标本。从组织标本中分离RNA并合成cDNA。我们考虑了定量实时PCR（qRT-PCR）来检测细胞内lncRNA的表达水平。此外，还评估了细胞因子的生物标志物活性以及lncRNA与临床病理特征之间的关系。结果：与非癌组织相比，癌组织中的CYTOR表达显著增加（P＜0.0001）。CYTOR表达与宫颈癌症鳞状细胞亚型之间存在显著相关性（P＝0.046）。受试者操作特征（ROC）曲线相关AUC（曲线下面积）、特异性和敏感性计算为0.88，81.74%和80%，这可能引入CYTOR作为潜在的生物标志物。结论：CYTOR在人宫颈癌症组织中表达增强，可能是癌症患者的有效癌基因和生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.80

自引率

23.50%

发文量

审稿时长

10 weeks

期刊介绍： The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.