Effect of Gallic Acid Pretreatment and SGK1 Enzyme Inhibition on Cardiac Function and Inflammation in a Rat Model of Ischemia-Reperfusion Injury.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Faramarz Souri, Mohammad Badavi, Mahin Dianat, Seyyed Ali Mard, Alireza Sarkaki
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引用次数: 0

Abstract

Background Serum and glucocorticoid-induced kinase 1 (SGK1) is an enzyme that may play an important role in ischemic-reperfusion (I/R) injury and myocardial dysfunction. Although many studies have been conducted on individual antioxidants, little attention has been paid to the effects of co-administration of an antioxidant with an SGK1 inhibitor on cardiac function after I/R. Methods This study aimed to determine the effects of gallic acid (as an antioxidant) combined with an SGK1 inhibitor on I/R-induced cardiac dysfunction and inflammation. Sixty male Wistar rats were randomized into 6 groups, pretreated with gallic acid or vehicle for 10 days. Subsequently, the heart was isolated and exposed to I/R. In groups that received the SGK1 inhibitor, the heart was perfused with the SGK1 inhibitor GSK650394, 5 min before induction of ischemia. After that, cardiac function, inflammatory factors, and myocardial damage were evaluated. Results The combination of these two compounds improved cardiac contractility, heart rate, rate pressure product, left ventricular developed pressure, left ventricular systolic pressure, perfusion pressure, and QRS voltage significantly (P < 0.05). In addition, concomitant therapy of these two agents reduced tumor necrosis factor-alpha and interleukin-6, and the activity of creatine kinase-MB, lactate dehydrogenase, and troponin-I (P < 0.05). Conclusion The results indicated that administration of gallic acid with the SGK1 inhibitor may have a potentiating effect on the improvement of cardiac dysfunction and I/R-induced inflammation.
没食子酸预处理和SGK1酶抑制对大鼠缺血再灌注损伤模型中心功能和炎症的影响。
背景:血清和糖皮质激素诱导激酶1(SGK1)是一种可能在缺血再灌注(I/R)损伤和心肌功能障碍中发挥重要作用的酶。尽管已经对单独的抗氧化剂进行了许多研究,但很少关注抗氧化剂与SGK1抑制剂联合给药对I/R后心脏功能的影响。将60只雄性Wistar大鼠随机分为6组,用没食子酸或赋形剂预处理10天。随后,心脏被隔离并暴露于I/R。在接受SGK1抑制剂的组中,在诱导缺血前5分钟,用SGK1抑制剂GSK650394灌注心脏。之后,评估心脏功能、炎症因子和心肌损伤。结果:这两种药物联合治疗可显著改善心肌收缩力、心率、率压积、左心室发展压、左心室收缩压、灌注压和QRS波电压(P<0.05)。此外,这两种制剂联合治疗可降低肿瘤坏死因子α和白细胞介素-6,降低肌酸激酶MB的活性,结论:没食子酸联合SGK1抑制剂可能对改善心功能障碍和I/R诱导的炎症有增强作用。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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