Physicochemical and bioinformatic characterization of Oreochromis niloticus vitellogenin as an endocrine disruption biomarker.

IF 2.4 4区环境科学与生态学 Q2 ECOLOGY

Ecotoxicology Pub Date : 2023-01-01 DOI:10.1007/s10646-022-02612-9

Tugstênio L Souza, Tobias P de Morais, Francisco Filipak Neto, Iracema Opuskevitch, Fernando C A S Ferreira, Marco Antônio Ferreira Randi, Ciro Alberto de Oliveira Ribeiro, Claudemir de Souza, Maritana Mela Prodocimo

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引用次数: 0

Abstract

Aquatic biota is increasingly being exposed to chemical pollutants due to human activities and the relationship between the level of environmental pollution and fish reproduction is a continuously ongoing issue. The vitellogenin (Vtg) protein synthesis can be induced in the liver of juvenile and male fish after stimulation of the estrogen receptor and therefore, Vtg has been used as a biomarker of xenoestrogen exposure in several fish species. The current study reported the first physicochemical characterization of Vtg from Oreochromis niloticus. Adult male fish were exposed to 17α-ethinylestradiol for Vtg induction. Purified vitellogenin from plasma showed low stability at 25 and 4 °C in saline conditions, and good stability in acidic (low pH) or in heated conditions. The 3D modeling provided useful information on the structure of O. niloticus Vtg showing conserved structural features. According to bioinformatics and experimental results, there are important structural differences between the two chemical forms of Vtg (VtgAb and VtgC) in a phylogenetic context. The present results add information about the development of ecotoxicological immunoassays to study the endocrine disruption in O. niloticus improving the Vtg performance as a biomarker of reproduction in fish.

查看原文本刊更多论文

nilochromis卵黄原蛋白作为内分泌干扰生物标志物的理化和生物信息学特征。

由于人类活动，水生生物群越来越多地暴露于化学污染物中，环境污染水平与鱼类繁殖之间的关系是一个持续存在的问题。卵黄原蛋白(vitellogenin, Vtg)蛋白在雌性激素受体的刺激下可在幼鱼和雄鱼的肝脏中被诱导合成，因此，Vtg已被用作几种鱼类的异雌性激素暴露的生物标志物。本研究首次报道了从尼罗褐藻中提取的Vtg的理化性质。将成年雄鱼暴露于17α-炔雌醇中诱导Vtg。血浆中纯化的卵黄原蛋白在25°C和4°C的生理盐水条件下稳定性较低，在酸性(低pH值)或加热条件下稳定性较好。三维建模为O. niloticus Vtg的结构提供了有用的信息，显示了保守的结构特征。根据生物信息学和实验结果，Vtg的两种化学形态(VtgAb和VtgC)在系统发育背景下存在重要的结构差异。本研究结果为生态毒理学免疫分析的发展提供了信息，以研究niloticus的内分泌干扰，提高Vtg性能作为鱼类繁殖的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ecotoxicology 环境科学-毒理学

CiteScore

5.30

自引率

3.70%

发文量

107

审稿时长

4.7 months

期刊介绍： Ecotoxicology is an international journal devoted to the publication of fundamental research on the effects of toxic chemicals on populations, communities and terrestrial, freshwater and marine ecosystems. It aims to elucidate mechanisms and processes whereby chemicals exert their effects on ecosystems and the impact caused at the population or community level. The journal is not biased with respect to taxon or biome, and papers that indicate possible new approaches to regulation and control of toxic chemicals and those aiding in formulating ways of conserving threatened species are particularly welcome. Studies on individuals should demonstrate linkage to population effects in clear and quantitative ways. Laboratory studies must show a clear linkage to specific field situations. The journal includes not only original research papers but technical notes and review articles, both invited and submitted. A strong, broadly based editorial board ensures as wide an international coverage as possible.