Egg yolk oil accelerates wound healing in streptozotocin induced diabetic rats.

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnic & Histochemistry Pub Date : 2023-02-01 DOI:10.1080/10520295.2022.2115554

Pinar Ili, Fikret Sari

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引用次数: 0

Abstract

Impaired diabetic wound healing causes foot ulcers. We investigated egg yolk oil for skin wound healing in streptozotocin (STZ) induced diabetic rats. Rats were allocated into three groups of six. Group 1, nondiabetic control group, was treated topically with 2% fusidic acid ointment. Group 2, STZ diabetic control, was treated topically with 2% fusidic acid ointment. Group 3, STZ diabetic group, was treated topically with egg yolk oil. Three days after STZ injection, two full thickness excisional skin wounds were created on the back of each animal. Wound diameter was measured for 14 days and wound contraction was calculated. Re-epithelization time also was determined. Three rats from each group were sacrificed on experimental day 7 and the remaining rats on day 14. Wound samples were examined using hematoxylin and eosin, periodic acid-Schiff, Masson's trichrome, Taenzer-Unna orcein and toluidine blue staining. Expression of endoglin (CD105), epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) were investigated using immunohistochemistry. Egg yolk oil increased the proliferation of epithelial cells and angiogenesis, and stimulated collagen deposition in the lesion area. Egg yolk oil increased CD105, EGF and VEGF expression in blood vessels, and EGF and VEGF expression in epidermis of the lesions. The predominant fatty acids in egg yolk oil are oleic, palmitic and linoleic, which likely were responsible for the beneficial effects of egg yolk oil on diabetic wound healing. Egg yolk oil appears to be a promising therapeutic agent for healing of diabetic wounds.

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蛋黄油促进链脲佐菌素诱导的糖尿病大鼠伤口愈合。

糖尿病伤口愈合受损会导致足部溃疡。研究了蛋黄油对链脲佐菌素(STZ)诱导的糖尿病大鼠皮肤创面愈合的影响。大鼠被分成三组，每组6只。第1组为非糖尿病对照组，采用2%夫西地酸软膏局部治疗。2组为STZ型糖尿病对照组，局部应用2%福西地酸软膏治疗。第三组为STZ糖尿病组，局部应用蛋黄油治疗。注射STZ 3天后，在每只动物背部做2个全层皮肤切口。第14天测量创面直径，计算创面收缩。再上皮化时间也被确定。实验第7天，每组处死3只大鼠，第14天处死其余大鼠。伤口样品采用苏木精和伊红、周期性酸-希夫、马松三色、Taenzer-Unna染色和甲苯胺蓝染色进行检测。免疫组化法检测内皮素(CD105)、表皮生长因子(EGF)和血管内皮生长因子(VEGF)的表达。蛋黄油增加了上皮细胞的增殖和血管生成，刺激了病变区域的胶原沉积。蛋黄油增加了血管中CD105、EGF和VEGF的表达以及病变表皮中EGF和VEGF的表达。蛋黄油中的主要脂肪酸是油酸、棕榈酸和亚油酸，这可能是蛋黄油对糖尿病伤口愈合有益的原因。蛋黄油是一种很有前途的治疗糖尿病伤口的药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnic & Histochemistry 生物-生物工程与应用微生物

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.