Genetic diversity of Staphylococcus aureus isolated from ear infections in Iran: Emergence of CC8/ST239-SCCmec III as major genotype.

IF 1.3 4区医学 Q4 IMMUNOLOGY

Acta microbiologica et immunologica Hungarica Pub Date : 2023-09-18 Print Date: 2023-09-21 DOI:10.1556/030.2023.02081

Zahra Rahmani, Sareh Sadat Hosseini, Parmida Bagheri, Mehdi Goudarzi

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Abstract

Increase in antibiotic resistance in Staphylococcus aureus isolated from ear infection is a serious public health problem. The objective of this investigation was to determine the antibacterial resistance profile and genetic variability of the S. aureus isolated from adult patients with otitis externa (OE) and otitis media (OM) infections, Tehran- Iran. The disk diffusion was employed to detect the susceptibility of 45 S. aureus strains. Biofilm production was evaluated by microtiter plate assay. Genetic diversity of the isolates was determined by staphylococcal cassette SCCmec, spa, and MLST techniques. Resistance to mupirocin and vancomycin were identified in 40 and 2.2% of isolates. Out of the 45 S. aureus isolates, 41 (91.2%) strains were considered as positive biofilm strains at different levels. According to our results, S. aureus isolated from OM (44.4%, 20/45) were including CC8/ST239-SCCmecIII corresponded to spa types t860, t030, t037, t234, t421 (70%, 14/20) and CC/ST30-SCCmecIV corresponded to spa types t605 and t019 (30%, 6/20) while S. aureus isolated from OE (55.6%, 25/45) were including CC/ST30-SCCmecIV corresponded to spa types t605, t345 and t1130 (52%, 13/25), CC/ST22-SCCmecIV corresponded to spa type t790 (20%, 5/25), CC8/ST8-SCCmecIV corresponded to spa type t008 (16%, 4/25), and CC/ST45-SCCmecIV corresponded to spa types t004 and t038 (12%, 3/25). This study highlighted genetic variability and strong biofilm formation ability among our isolates revealing its crucial role in enhancing the resistance of this bacteria to drugs. Thus, it is necessary to continue the epidemiological analysis to improve the control of ear infections related to S. aureus.

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从伊朗耳部感染中分离的金黄色葡萄球菌的遗传多样性：CC8/ST239 SCCmec III作为主要基因型的出现。

从耳朵感染中分离出的金黄色葡萄球菌的抗生素耐药性增加是一个严重的公共卫生问题。本研究的目的是确定从伊朗德黑兰外耳道炎（OE）和中耳炎（OM）感染的成年患者中分离的金黄色葡萄球菌的抗菌耐药性和遗传变异性。采用纸片扩散法对45株金黄色葡萄球菌进行了药敏试验。通过微量滴定板测定法评估生物膜的产生。通过葡萄球菌盒SCCmec、spa和MLST技术测定分离株的遗传多样性。在40个和2.2%的分离株中鉴定出对莫匹罗星和万古霉素的耐药性。在45株金黄色葡萄球菌分离株中，41株（91.2%）被认为是不同水平的生物膜阳性菌株。根据我们的结果，从OM中分离的金黄色葡萄球菌（44.4%，20/45）包括与spa类型t860、t030、t037、t234、t421（70%，14/20）相对应的CC8/ST239 SCCmecIII，并且CC/ST30 SCCmecIV与spa型t605和t019（30%，6/20）相对应，而从OE中分离的黄金黄色葡萄球菌则包括与spa类型t605、t345和t1130相对应的CC/ST30 SCCmecIV（52%，13/25），CC/ST22 SCCmecIV对应spa类型t790（20%，5/25），CC8/ST8 SCCmecⅣ对应spa型t008（16%，4/25），而CC/ST45 SCCmecIV对应spa类t004和t038（12%，3/25）。这项研究强调了我们分离株的遗传变异性和强大的生物膜形成能力，揭示了它在增强这种细菌耐药性方面的关键作用。因此，有必要继续进行流行病学分析，以改善与金黄色葡萄球菌相关的耳部感染的控制。

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来源期刊

Acta microbiologica et immunologica Hungarica IMMUNOLOGY-MICROBIOLOGY

CiteScore

2.30

自引率

13.30%

发文量

审稿时长

>12 weeks

期刊介绍： AMIH is devoted to the publication of research in all fields of medical microbiology (bacteriology, virology, parasitology, mycology); immunology of infectious diseases and study of the microbiome related to human diseases.