Effect of ferulic acid on testicular damage caused by torsion-detorsion in rats.

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnic & Histochemistry Pub Date : 2023-02-01 DOI:10.1080/10520295.2022.2110615

Uygar Saçık, Zahide Çavdar, Cemre Ural, Nevin Ersoy, Candan Özoğul, Güven Erbil

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引用次数: 2

Abstract

Testicular torsion is twisting of the spermatic cord around its axis, which impairs blood flow and causes ischemia and formation of free radicals. Ferulic acid is a phenolic acid of the hydroxycinnamic family that is found in the seeds and leaves of plants; it is present in substantial amounts in fruits and vegetables. We investigated the protective effect of ferulic acid on experimental testicular torsion in rats. Animals were divided randomly into five groups: control, ethyl alcohol, torsion, torsion-detorsion, and torsion-detorsion + ferulic acid. Histopathology was assessed using hematoxylin and eosin, and periodic acid-Schiff staining. Tissues were assessed using TUNEL, active caspase-3, myeloperoxidase and inducible nitric oxide synthase immunostaining. Biochemical changes were assessed using assays for superoxide dismutase, malondialdehyde, glutathione peroxidase and glutathione. Ferulic acid reduced the levels of free radicals and increased the levels of antioxidants. Ferulic acid also reduced histopathological changes and germ cell differentiation in the testis following torsion-detorsion. Ferulic acid should be investigated further as a potential treatment for sequelae of torsion-detorsion injury.

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阿魏酸对大鼠睾丸扭扭损伤的影响。

睾丸扭转是指精索绕其轴扭曲，影响血液流动，引起缺血和自由基的形成。阿魏酸是羟基肉桂酸家族的一种酚酸，存在于植物的种子和叶子中;它大量存在于水果和蔬菜中。研究阿魏酸对大鼠实验性睾丸扭转的保护作用。动物随机分为5组:对照组、乙醇组、扭转组、扭转-扭转组、扭转-扭转+阿魏酸组。组织病理学采用苏木精和伊红，定期酸-希夫染色。采用TUNEL、活性caspase-3、髓过氧化物酶和诱导型一氧化氮合酶免疫染色对组织进行评估。采用超氧化物歧化酶、丙二醛、谷胱甘肽过氧化物酶和谷胱甘肽检测来评估生化变化。阿魏酸降低了自由基的水平，增加了抗氧化剂的水平。阿魏酸还能降低睾丸扭转-扭转后的组织病理学改变和生殖细胞分化。阿魏酸作为扭转-扭转损伤后遗症的潜在治疗方法有待进一步研究。

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来源期刊

Biotechnic & Histochemistry 生物-生物工程与应用微生物

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.