Role of phosphatidylserine in the localization of cell surface membrane proteins in yeast.

IF 2 4区 生物学 Q4 CELL BIOLOGY
Cell structure and function Pub Date : 2023-01-28 Epub Date: 2022-12-15 DOI:10.1247/csf.22081
Ryutaro Kashikuma, Makoto Nagano, Hiroki Shimamura, Kouya Nukaga, Ikumi Katsumata, Junko Y Toshima, Jiro Toshima
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引用次数: 1

Abstract

Phosphatidylserine (PS) is a constituent of the cell membrane, being especially abundant in the cytoplasmic leaflet, and plays important roles in a number of cellular functions, including the formation of cell polarity and intracellular vesicle transport. Several studies in mammalian cells have suggested the role of PS in retrograde membrane traffic through endosomes, but in yeast, where PS is localized primarily at the plasma membrane (PM), the role in intracellular organelles remains unclear. Additionally, it is reported that polarized endocytic site formation is defective in PS-depleted yeast cells, but the role in the endocytic machinery has not been well understood. In this study, to clarify the role of PS in the endocytic pathway, we analyzed the effect of PS depletion on endocytic internalization and post-endocytic transport. We demonstrated that in cell lacking the PS synthase Cho1p (cho1Δ cell), binding and internalization of mating pheromone α-factor into the cell was severely impaired. Interestingly, the processes of endocytosis were mostly unaffected, but protein transport from the trans-Golgi network (TGN) to the PM was defective and localization of cell surface proteins was severely impaired in cho1Δ cells. We also showed that PS accumulated in intracellular compartments in cells lacking Rcy1p and Vps52p, both of which are implicated in endosome-to-PM transport via the TGN, and that the number of Snx4p-residing endosomes was increased in cho1Δ cells. These results suggest that PS plays a crucial role in the transport and localization of cell surface membrane proteins.Key words: phosphatidylserine, endocytosis, recycling, vesicle transport.

磷脂酰丝氨酸在酵母细胞表面膜蛋白定位中的作用
磷脂酰丝氨酸(PS)是细胞膜的一种成分,在细胞质小叶中含量特别丰富,在细胞极性形成和细胞内囊泡运输等多种细胞功能中发挥着重要作用。在哺乳动物细胞中进行的一些研究表明,PS 在通过内体的逆行膜运输中发挥作用,但在酵母中,PS 主要定位于质膜(PM),在细胞内细胞器中的作用仍不清楚。此外,有报道称,在缺失 PS 的酵母细胞中,极化内吞部位的形成是有缺陷的,但其在内吞机中的作用还不是很清楚。在本研究中,为了明确 PS 在内吞膜途径中的作用,我们分析了 PS 缺失对内吞膜内化和内吞膜后运输的影响。我们发现,在缺乏 PS 合成酶 Cho1p 的细胞(cho1Δ 细胞)中,交配信息素 α-因子与细胞的结合和内化受到严重影响。有趣的是,在 cho1Δ 细胞中,内吞过程大多不受影响,但蛋白质从跨高尔基体网络(TGN)向 PM 的转运存在缺陷,细胞表面蛋白质的定位严重受损。我们还发现,在缺乏 Rcy1p 和 Vps52p 的细胞中,PS 在细胞内积累,而这两种物质都与通过 TGN 的内体细胞到 PM 的转运有关;在 cho1Δ 细胞中,Snx4p 驻留内体的数量增加。这些结果表明,PS在细胞表面膜蛋白的转运和定位中起着至关重要的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell structure and function
Cell structure and function 生物-细胞生物学
CiteScore
2.50
自引率
0.00%
发文量
6
审稿时长
>12 weeks
期刊介绍: Cell Structure and Function is a fully peer-reviewed, fully Open Access journal. As the official English-language journal of the Japan Society for Cell Biology, it is published continuously online and biannually in print. Cell Structure and Function publishes important, original contributions in all areas of molecular and cell biology. The journal welcomes the submission of manuscripts on research areas such as the cell nucleus, chromosomes, and gene expression; the cytoskeleton and cell motility; cell adhesion and the extracellular matrix; cell growth, differentiation and death; signal transduction; the protein life cycle; membrane traffic; and organelles.
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