Investigating DNA barcodes of plants growing in some areas of Iran with high crime rate: Quercus brantii, Curpressus arizonica, Crataegus pentagyna, Ziziphus Spina-chtista, and Buxus hyrcana

IF 1.9 4区医学 Q2 MEDICINE, LEGAL

Science & Justice Pub Date : 2023-09-01 DOI:10.1016/j.scijus.2023.07.006

Sajadian Abdollah , Zamanizadeh Hamid Reza , Akhavan Sepahi Abbas , Amani jafar

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引用次数: 0

Abstract

According to criminal botany, the offender unknowingly carries plant samples from the crime scene. Therefore, studying the genetic data of plants native to the crime scene can solve many ambiguities in the criminal files. In this regard, the aim of this study was to investigate the genome of 5 endemic plants in some areas of Iran with high crime rate. Quercus brantii, Curpressus arizonica, Crataegus pentagyna, Ziziphus Spina-chtista, and Buxus hyrcana were assessed using 1 genetic fragment on plastid regions (trnH-psbA) as well as 1 gene on nuclear chromosome called ITS. The alignment of DNA sequences of trnH-psbA and ITS genes was done using BioEdit, Clustal X, and Muscle v4.0 software programs. The phylogenetic analysis was performed on aligned data using Maximum Parsimony (MP) and the Bayesian methods. The Splits Tree v.4.14.4 software program was used for phylogenetic network analysis. Finally, the data combinability test was conducted using the Incongruence Length Difference (ILD) test by PAUP* software program. All data from nrDNA ITS and trnH-psbA sequences were consistent with Information Compatibility Test (ICT) results. Moreover, the nrDNA ITS indicated more resolved relationship than trnH-psbA. The results from MP and Bayesian analyses did not differ significantly between singular and combined forms, except for a slight variance in confidence interval of branches. As the phylogenetic trees provide more thorough and deeper conception of species relations, it is hoped that they would be useful to illuminate some forensic gaps in regions with high crime rates enriched by these plants, not only in Iran, but also in all areas over the world with this vegetation.

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调查生长在伊朗一些犯罪率高的地区的植物的DNA条形码：白栎、亚利桑那Curpressus arizonica、五女山楂、刺酸枣和黄杨。

根据犯罪植物学，犯罪者在不知情的情况下携带犯罪现场的植物样本。因此，研究犯罪现场原生植物的基因数据可以解决犯罪档案中的许多歧义。在这方面，本研究的目的是调查伊朗一些犯罪率高的地区的5种特有植物的基因组。使用质体区域上的1个遗传片段（trnH-psbA）和核染色体上的1种称为ITS的基因评估了布兰栎、亚利桑那Curpressus arizonica、五核山楂、刺酸枣和黄杨。使用BioEdit、Clustal X和Muscle v4.0软件程序对trnH-psbA和ITS基因的DNA序列进行比对。使用最大分析（MP）和贝叶斯方法对比对数据进行系统发育分析。Splits-Tree 4.14.4版软件程序用于系统发育网络分析。最后，利用PAUP*软件程序进行了不一致长度差（ILD）测试，对数据的可组合性进行了测试。来自nrDNA-ITS和trnH-psbA序列的所有数据与信息兼容性测试（ICT）结果一致。此外，与trnH-psbA相比，nrDNA-ITS显示出更多的解析关系。MP和贝叶斯分析的结果在奇异形式和组合形式之间没有显著差异，只是分支的置信区间略有差异。随着系统发育树对物种关系提供了更彻底、更深入的概念，人们希望它们将有助于阐明这些植物富集的高犯罪率地区的一些法医学空白，不仅在伊朗，而且在世界上所有有这种植被的地区。

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来源期刊

Science & Justice 医学-病理学

CiteScore

4.20

自引率

15.80%

发文量

审稿时长

81 days

期刊介绍： Science & Justice provides a forum to promote communication and publication of original articles, reviews and correspondence on subjects that spark debates within the Forensic Science Community and the criminal justice sector. The journal provides a medium whereby all aspects of applying science to legal proceedings can be debated and progressed. Science & Justice is published six times a year, and will be of interest primarily to practising forensic scientists and their colleagues in related fields. It is chiefly concerned with the publication of formal scientific papers, in keeping with its international learned status, but will not accept any article describing experimentation on animals which does not meet strict ethical standards. Promote communication and informed debate within the Forensic Science Community and the criminal justice sector. To promote the publication of learned and original research findings from all areas of the forensic sciences and by so doing to advance the profession. To promote the publication of case based material by way of case reviews. To promote the publication of conference proceedings which are of interest to the forensic science community. To provide a medium whereby all aspects of applying science to legal proceedings can be debated and progressed. To appeal to all those with an interest in the forensic sciences.