From clean spaces to crime scenes: Exploring trace DNA recovery from titania-coated self-cleaning substrates

IF 1.9 4区医学 Q2 MEDICINE, LEGAL

Science & Justice Pub Date : 2023-09-01 DOI:10.1016/j.scijus.2023.07.003

Dan Nana Osei Bonsu , Denice Higgins , Jeremy J. Austin

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引用次数: 0

Abstract

Titanium dioxide (titania, TiO₂) is frequently used as a coating for a variety of self-cleaning products, such as antifogging vehicle mirrors, ceramic tiles, and glass windows because of its distinct physiochemical features. When exposed to light TiO₂ causes photocatalytic decomposition of organic contaminants, potentially compromising DNA integrity. The impact of TiO₂-coated commercial glasses, Bioclean® and SaniTise™, on trace DNA persistence, recovery, and profiling was investigated. DNA in saliva and touch samples deposited on self-cleaning glass slides exposed to indoor fluorescent light for up to seven days was more degraded than control samples indicating some degree of fluorescent light-induced photocatalytic activity of the self-cleaning surfaces. When exposed to sunlight, DNA yields from saliva and touch samples deposited on the titania-coated substrates decreased rapidly, with a corresponding increase in DNA degradation. After three days no DNA samples applied to self-cleaning glass and exposed to natural sunlight yielded STR profiles. These results suggest that the photocatalytic activation of TiO₂ is the likely mechanism of action underlying the extreme DNA degradation on the Bioclean® and SaniTise™ glasses. Consequently, rapid sample collection and use may be warranted in casework scenarios involving TiO₂-coated materials.

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从清洁空间到犯罪现场：探索从二氧化钛涂层的自清洁基质中回收微量DNA。

二氧化钛（二氧化钛，TiO2）由于其独特的物理化学特性，经常被用作各种自清洁产品的涂层，如防雾车镜、瓷砖和玻璃窗。当暴露在光下时，TiO2会导致有机污染物的光催化分解，可能会损害DNA的完整性。TiO2涂层商用玻璃、Bioclean®和SaniTise的影响™, 对微量DNA的持久性、回收率和图谱进行了研究。沉积在暴露于室内荧光长达七天的自清洁载玻片上的唾液和触摸样品中的DNA比对照样品降解得更多，这表明自清洁表面具有一定程度的荧光诱导的光催化活性。当暴露在阳光下时，沉积在二氧化钛涂层基底上的唾液和触摸样本的DNA产量迅速下降，DNA降解相应增加。三天后，没有DNA样本应用于自清洁玻璃并暴露于自然阳光下产生STR图谱。这些结果表明，TiO2的光催化活化可能是Bioclean®和SaniTise极端DNA降解的潜在作用机制™ 玻璃杯因此，在涉及TiO2涂层材料的个案工作场景中，可以保证快速收集和使用样品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science & Justice 医学-病理学

CiteScore

4.20

自引率

15.80%

发文量

审稿时长

81 days

期刊介绍： Science & Justice provides a forum to promote communication and publication of original articles, reviews and correspondence on subjects that spark debates within the Forensic Science Community and the criminal justice sector. The journal provides a medium whereby all aspects of applying science to legal proceedings can be debated and progressed. Science & Justice is published six times a year, and will be of interest primarily to practising forensic scientists and their colleagues in related fields. It is chiefly concerned with the publication of formal scientific papers, in keeping with its international learned status, but will not accept any article describing experimentation on animals which does not meet strict ethical standards. Promote communication and informed debate within the Forensic Science Community and the criminal justice sector. To promote the publication of learned and original research findings from all areas of the forensic sciences and by so doing to advance the profession. To promote the publication of case based material by way of case reviews. To promote the publication of conference proceedings which are of interest to the forensic science community. To provide a medium whereby all aspects of applying science to legal proceedings can be debated and progressed. To appeal to all those with an interest in the forensic sciences.