Research Development on Exosome Separation Technology.

IF 2.3 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Membrane Biology Pub Date : 2023-02-01 DOI:10.1007/s00232-022-00260-y

Wei-Ming Xu, Ao Li, Jia-Jun Chen, En-Jie Sun

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引用次数: 13

Abstract

Exosomes are special extracellular vesicles secreted by cells, which are of great significance in the basic research of life science and clinical application and has become a hot research field with rapid development in recent 10 years. Therefore, the isolation and separation of exosomes is particularly important for the research and application of exosomes. This paper aims to review the research progress of exosome isolation and separation methods in recent years, including ultracentrifugation, ultrafiltration, size‑exclusion chromatography, precipitation, immunomagnetic bead capture method, aptamer-based isolation, and isolation methods based on microfluidic technology. It is generally accepted that most of the existing methods have limitations, for example, ultracentrifugation is time-consuming and laborious, and immunomagnetic bead capture method and aptamer-based separation method have small sample processing capacity and high cost. As a result, we also introduce some common situations in which two or more methods are combined for use. Finally, the separation and isolation methods including all those presented in this review were compared and summarized.

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外泌体分离技术的研究进展。

外泌体是细胞分泌的一种特殊的细胞外囊泡，在生命科学的基础研究和临床应用中具有重要意义，是近10年来快速发展的一个研究热点。因此，外泌体的分离和分离对于外泌体的研究和应用尤为重要。本文综述了近年来外泌体分离方法的研究进展，包括超离心、超滤、排粒径层析、沉淀法、免疫磁珠捕获法、基于适体的分离方法、基于微流控技术的分离方法等。人们普遍认为，现有的方法大多存在局限性，如超离心费时费力，免疫磁珠捕获法和基于适体的分离法样品处理能力小，成本高。因此，我们还介绍了将两个或多个方法组合使用的一些常见情况。最后，对本文介绍的几种分离分离方法进行了比较和总结。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Membrane Biology 生物-生化与分子生物学

CiteScore

4.80

自引率

4.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Membrane Biology is dedicated to publishing high-quality science related to membrane biology, biochemistry and biophysics. In particular, we welcome work that uses modern experimental or computational methods including but not limited to those with microscopy, diffraction, NMR, computer simulations, or biochemistry aimed at membrane associated or membrane embedded proteins or model membrane systems. These methods might be applied to study topics like membrane protein structure and function, membrane mediated or controlled signaling mechanisms, cell-cell communication via gap junctions, the behavior of proteins and lipids based on monolayer or bilayer systems, or genetic and regulatory mechanisms controlling membrane function. Research articles, short communications and reviews are all welcome. We also encourage authors to consider publishing ''negative'' results where experiments or simulations were well performed, but resulted in unusual or unexpected outcomes without obvious explanations. While we welcome connections to clinical studies, submissions that are primarily clinical in nature or that fail to make connections to the basic science issues of membrane structure, chemistry and function, are not appropriate for the journal. In a similar way, studies that are primarily descriptive and narratives of assays in a clinical or population study are best published in other journals. If you are not certain, it is entirely appropriate to write to us to inquire if your study is a good fit for the journal.