In vivo application of magnetic molecularly imprinted polymer in rheumatoid arthritis rat model.

IF 4.3 4区医学 Q1 PHARMACOLOGY & PHARMACY

Journal of Drug Targeting Pub Date : 2023-09-01 DOI:10.1080/1061186X.2023.2247584

Mariam Soliman, Nagwan Shanan, Gamal Eissa, Boris Mizaikoff, Nesrine A El Gohary

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Abstract

Abstract A magnetic molecularly imprinted polymer (MMIP) was synthesised and tested for an in vivo rheumatoid arthritis (RA) rat model. Magnetite coated with mesoporous silica (Fe2O3@mSi) was used as core for surface imprinting, dopamine was used as monomer and methotrexate (MTX) was loaded directly during polymerisation. The amount of MTX loaded on MMIPs reached 201.165 ± 0.315 µmol/g. Characterisation of the polymers was done via SEM, TEM, and FTIR. The pharmacological effect of the selected MMIP was evaluated in a Complete Freund’s Adjuvant (CFA) induced arthritis rat model where a 3D magnet bearing construct was designed for targeted delivery of MMIPs. The parameters evaluated were the change in paw edoema, paw diameter, gait score, and animal’s weight. Results revealed a tendency of MMIP to significantly improve the measured parameters which was confirmed with histopathological findings. In conclusion, the improvement in the arthritic signs associated with MMIP treatment compared to free MTX, indicated successful targeting of MMIPs to the site of inflammation. Graphical Abstract

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磁性分子印迹聚合物在类风湿关节炎大鼠模型中的体内应用。

合成了磁性分子印迹聚合物(MMIP)，并对其在类风湿性关节炎(RA)大鼠体内模型中的应用进行了实验。以介孔二氧化硅(Fe2O3@mSi)包裹的磁铁矿作为表面印迹的核心，以多巴胺为单体，在聚合过程中直接加载甲氨蝶呤(MTX)。MTX在MMIPs上的负载量为201.165±0.315µmol/g。通过SEM, TEM和FTIR对聚合物进行了表征。在完全弗氏佐剂(CFA)诱导的关节炎大鼠模型中评估所选MMIPs的药理作用，其中设计了一个3D磁铁轴承结构用于靶向递送MMIPs。评估的参数是脚掌水肿、脚掌直径、步态评分和动物体重的变化。结果显示，MMIP有显著改善测量参数的趋势，这与组织病理学结果一致。总之，与游离MTX相比，MMIPs治疗相关的关节炎症状的改善表明MMIPs成功靶向炎症部位。

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来源期刊

Journal of Drug Targeting 医学-药学

CiteScore

9.10

自引率

0.00%

发文量

165

审稿时长

2 months

期刊介绍： Journal of Drug Targeting publishes papers and reviews on all aspects of drug delivery and targeting for molecular and macromolecular drugs including the design and characterization of carrier systems (whether colloidal, protein or polymeric) for both vitro and/or in vivo applications of these drugs. Papers are not restricted to drugs delivered by way of a carrier, but also include studies on molecular and macromolecular drugs that are designed to target specific cellular or extra-cellular molecules. As such the journal publishes results on the activity, delivery and targeting of therapeutic peptides/proteins and nucleic acids including genes/plasmid DNA, gene silencing nucleic acids (e.g. small interfering (si)RNA, antisense oligonucleotides, ribozymes, DNAzymes), as well as aptamers, mononucleotides and monoclonal antibodies and their conjugates. The diagnostic application of targeting technologies as well as targeted delivery of diagnostic and imaging agents also fall within the scope of the journal. In addition, papers are sought on self-regulating systems, systems responsive to their environment and to external stimuli and those that can produce programmed, pulsed and otherwise complex delivery patterns.

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