Specnuezhenide ameliorates ultraviolet-induced skin photoaging in mice by regulating the Sirtuin 3/8-Oxoguanine DNA glycosylase signal.

IF 2.5 4区医学 Q2 DERMATOLOGY

Photodermatology, photoimmunology & photomedicine Pub Date : 2023-09-01 DOI:10.1111/phpp.12880

Nan Tang, Ying-Yun Ren, Hao-Tian Wu, Xi-Ting Lv, Xiao-Ting Liu, Qi-Lin Li, Guo-En Wang, Yan-Hua Wu

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引用次数: 1

Abstract

Purpose: Ultraviolet-induced skin photoaging was involved in DNA oxidative damage. Specnuezhenide, one of the secoiridoids extracted from Ligustri Lucidi Fructus, possesses antioxidant and anti-inflammatory effects. Whether specnuezhenide ameliorates skin photoaging remains unclear. This study aimed to investigate the effect of specnuezhenide on skin photoaging induced by ultraviolet and explore the underlying mechanism.

Methods: Mice were employed to treat with ultraviolet to induce skin photoaging, then administrated 10 and 20 mg/kg of specnuezhenide. Histological analysis, protein expression, network pharmacology, and autodock analysis were conducted.

Results: Specnuezhenide ameliorated ultraviolet-induced skin photoaging in mice via the increase in collagen contents, and decrease in epidermal thickness, malondialdehyde content, and β-galactosidase expression in the skin. Specnuezhenide reduced cutaneous apoptosis and inflammation in mice with skin photoaging. In addition, network pharmacology data indicated that specnuezhenide possessed potential targets on the NOD-like receptor signaling pathway. Validation experiment found that specnuezhenide inhibited the expression of NOD-like receptor family pyrin domain-containing 3, gasdermin D-C1, and Caspase 1. Furthermore, the expression of 8-Oxoguanine DNA glycosylase (OGG1), sirtuin 3 (SIRT3), and superoxide dismutase 2 was increased in specnuezhenide-treated mice with photoaging.

Conclusion: Specnuezhenide protected against ultraviolet-induced skin photoaging in mice via a probable activation of SIRT3/OGG1 signal.

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Specnuezhenide通过调节Sirtuin 3/8-氧鸟嘌呤DNA糖基酶信号改善紫外线诱导的小鼠皮肤光老化。

目的:紫外线诱导的皮肤光老化与DNA氧化损伤有关。山茱萸烯醚酯是一种从女贞子中提取的二环烯醚萜类化合物，具有抗氧化和抗炎作用。specnuezhenide是否能改善皮肤光老化尚不清楚。本研究旨在研究specnuezhenide对紫外光致皮肤光老化的影响，并探讨其作用机制。方法:用紫外光诱导小鼠皮肤光老化，然后分别给药10、20 mg/kg的specnuezhenide。进行组织学分析、蛋白表达、网络药理学和autodock分析。结果:Specnuezhenide通过增加皮肤胶原含量、降低皮肤表皮厚度、丙二醛含量和β-半乳糖苷酶表达来改善紫外线诱导的小鼠皮肤光老化。Specnuezhenide减少皮肤光老化小鼠的皮肤凋亡和炎症。此外，网络药理学数据表明，specnuezhenide在nod样受体信号通路上具有潜在的靶点。验证实验发现，specnuezhenide抑制nod样受体家族pyrin -containing 3、gasdermin D-C1和Caspase 1的表达。此外，8-氧鸟嘌呤DNA糖基化酶(OGG1)、sirtuin 3 (SIRT3)和超氧化物歧化酶2在specnuezhenide处理的光老化小鼠中表达增加。结论:Specnuezhenide可能通过激活SIRT3/OGG1信号来防止紫外线诱导的小鼠皮肤光老化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Photodermatology, photoimmunology & photomedicine 医学-皮肤病学

CiteScore

4.40

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal is a forum for new information about the direct and distant effects of electromagnetic radiation (ultraviolet, visible and infrared) mediated through skin. The divisions of the editorial board reflect areas of specific interest: aging, carcinogenesis, immunology, instrumentation and optics, lasers, photodynamic therapy, photosensitivity, pigmentation and therapy. Photodermatology, Photoimmunology & Photomedicine includes original articles, reviews, communications and editorials. Original articles may include the investigation of experimental or pathological processes in humans or animals in vivo or the investigation of radiation effects in cells or tissues in vitro. Methodology need have no limitation; rather, it should be appropriate to the question addressed.