Estradiol-17β [E2] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound model.

IF 6.7 1区 工程技术 Q1 CELL & TISSUE ENGINEERING
Sarah Shafaat, Sabiniano Roman Regueros, Christopher Chapple, Sheila MacNeil, Vanessa Hearnden
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Abstract

Childbirth contributes to common pelvic floor problems requiring reconstructive surgery in postmenopausal women. Our aim was to develop a tissue-engineered vaginal wound model to investigate wound healing and the contribution of estradiol to pelvic tissue repair. Partial thickness scalpel wounds were made in tissue models based on decellularized sheep vaginal matrices cultured with primary sheep vaginal epithelial cells and fibroblasts. Models were cultured at an airliquid interface (ALI) for 3 weeks with and without estradiol-17β [E2]. Results showed that E2 significantly increased wound healing and epithelial maturation. Also, E2 led to collagen reorganization after only 14 days with collagen fibers more regularly aligned and compactly arranged Additionally, E2 significantly downregulated α-SMA expression which is involved in fibrotic tissue formation. This model allows one to investigate multiple steps in vaginal wound healing and could be a useful tool in developing therapies for improved tissue healing after reconstructive pelvic floor surgery.

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雌二醇-17β [E2]在体外3D组织工程阴道伤口模型中刺激伤口愈合。
分娩有助于常见的盆底问题,需要重建手术在绝经后妇女。我们的目的是建立一个组织工程阴道伤口模型来研究伤口愈合和雌二醇对骨盆组织修复的贡献。以原代羊阴道上皮细胞和成纤维细胞培养的脱细胞羊阴道基质为基础,建立了部分厚度手术刀伤口的组织模型。模型在气液界面(ALI)培养3周,分别添加和不添加雌二醇-17β [E2]。结果显示E2显著促进创面愈合和上皮细胞成熟。E2仅在14天后就导致胶原蛋白重组,胶原纤维排列更加整齐和紧密。E2显著下调参与纤维化组织形成的α-SMA的表达。该模型允许研究阴道伤口愈合的多个步骤,并可能成为开发改善骨盆底重建手术后组织愈合的治疗方法的有用工具。
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来源期刊
Journal of Tissue Engineering
Journal of Tissue Engineering Engineering-Biomedical Engineering
CiteScore
11.60
自引率
4.90%
发文量
52
审稿时长
12 weeks
期刊介绍: The Journal of Tissue Engineering (JTE) is a peer-reviewed, open-access journal dedicated to scientific research in the field of tissue engineering and its clinical applications. Our journal encompasses a wide range of interests, from the fundamental aspects of stem cells and progenitor cells, including their expansion to viable numbers, to an in-depth understanding of their differentiation processes. Join us in exploring the latest advancements in tissue engineering and its clinical translation.
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