Activation of GRP78 ATPase suppresses A549 lung cancer cell migration by promoting ITGB4 degradation.

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Junya Ning, Xiaoling Cui, Nan Li, Na Li, Baoxiang Zhao, Junying Miao, Zhaomin Lin
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引用次数: 4

Abstract

Hypochlorous acid (HOCl) is an essential signal molecule in cancer cells. Activated GRP78 ATPase by a HOCl probe named ZBM-H inhibits lung cancer cell growth. However, the role and underlying mechanism of GRP78 ATPase in lung cancer cell migration have not been established. Here, we reported that activation of GRP78 ATPase by ZBM-H suppressed A549 cell migration and inhibited EMT process. Notably, ZBM-H time-dependently decreased the protein level of integrin β4 (ITGB4) in A549 cells. Combinatorial treatment of 3BDO (an autophagy inhibitor) and ZBM-H partially rescued the protein level of ITGB4. Consistently, 3BDO partially reversed ZBM-H-inhibited cell migration. Furthermore, ZBM-H promoted the interaction between ANXA7 and Hsc70, which participated in the regulation of selective autophagy and degradation of ITGB4.

Abstract Image

Abstract Image

Abstract Image

激活GRP78 atp酶通过促进ITGB4降解抑制A549肺癌细胞迁移。
次氯酸(HOCl)是肿瘤细胞中必不可少的信号分子。ZBM-H探针激活GRP78 atp酶抑制肺癌细胞生长。然而,GRP78 atp酶在肺癌细胞迁移中的作用和潜在机制尚未确定。在这里,我们报道了ZBM-H激活GRP78 atp酶抑制A549细胞迁移和抑制EMT过程。值得注意的是,ZBM-H具有时间依赖性,可降低A549细胞中整合素β4 (ITGB4)的蛋白水平。3BDO(一种自噬抑制剂)和ZBM-H联合治疗可部分恢复ITGB4蛋白水平。与此一致,3BDO部分逆转了zbm - h抑制的细胞迁移。ZBM-H促进ANXA7与Hsc70的相互作用,参与调控ITGB4的选择性自噬和降解。
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来源期刊
CiteScore
6.40
自引率
0.00%
发文量
7
审稿时长
53 weeks
期刊介绍: Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.
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