Involvement of Cannabinoid Type 2 Receptors in the Favorable Effects of Sumatriptan on the Random-Pattern Skin Flap Survival in Rats: A Novel Potential Target.

IF 1.7 4区医学 Q2 SURGERY

European Surgical Research Pub Date : 2022-01-01 DOI:10.1159/000521581

Armin Aryannejad, Nafise Noroozi, Seyed Mohammad Tavangar, Samira Ramezani, Amir Rashidian, Reza Laripour, Mojtaba Yousefi Zoshk, Ahmad Reza Dehpour, Mohsen Chamanara

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引用次数: 3

Abstract

Introduction: Recent investigations have indicated the potential therapeutic role of cannabinoid type 2 (CB2) receptors in various inflammatory-related disorders. However, the role of these receptors has not been studied in skin flap models to date. In this study, we aimed to evaluate the possible involvement of CB2 receptors in the anti-inflammatory effects of sumatriptan, improving the random-pattern skin flap survival in rats.

Methods: In a controlled experimental study, 36 male Wistar rats were randomly divided into 6 study groups (n = 6 per group). Two doses of sumatriptan (0.1 and 0.3 mg/kg) were administered intraperitoneally 30 min before harvesting the flap tissue. In a separate group, SR144528 (a selective CB2 receptor inverse agonist) was injected before the most effective dose of sumatriptan to determine the possible involvement of CB2 receptors in its action. Histopathological examinations, the expression level of CB2 receptors (Western blot analysis), and IL-1β and TNF-α concentrations (ELISA) were explored in the skin flap sampled tissues.

Results: Sumatriptan 0.3 mg/kg remarkably enhanced the skin flap survival in all macroscopic and microscopic investigations compared to the control group (p < 0.001). IL-1β and TNF-α levels were significantly attenuated (p < 0.001), and the expression of CB2 receptors in skin cells was amplified in rats treated with sumatriptan 0.3 mg/kg (p < 0.05) compared to the control group. However, the administration of SR144528 (2 mg/kg) nullified all the protective effects of sumatriptan 0.3 mg/kg.

Conclusion: We discovered that CB2 receptors play a crucial role in the favorable effects of sumatriptan on skin flap survival as a novel mechanism of action. So, targeting these receptors seems to be a dependable method in skin flap surgeries to ensure its survival and prevent tissue necrosis. Further experimental and clinical investigations are needed to ensure the safe clinical application of this method.

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大麻素2型受体参与舒马曲坦对大鼠随机皮瓣存活的有利作用:一个新的潜在靶点。

最近的研究表明大麻素2型(CB2)受体在各种炎症相关疾病中的潜在治疗作用。然而，这些受体的作用尚未在皮瓣模型中得到研究。在本研究中，我们旨在评估CB2受体可能参与舒马曲坦的抗炎作用，改善大鼠随机皮瓣存活。方法:采用对照实验方法，将36只雄性Wistar大鼠随机分为6个研究组，每组6只。在收获皮瓣组织前30分钟腹腔注射两剂舒马曲坦(0.1和0.3 mg/kg)。在另一组中，SR144528(一种选择性CB2受体逆激动剂)在服用最有效剂量的舒马普坦之前注射，以确定CB2受体可能参与其作用。组织病理学检查、CB2受体表达水平(Western blot)和IL-1β、TNF-α浓度(ELISA)。结果:与对照组相比，0.3 mg/kg舒马曲坦能显著提高皮瓣的宏观和微观存活率(p < 0.001)。与对照组相比，0.3 mg/kg舒马曲坦组大鼠皮肤细胞中IL-1β和TNF-α水平明显降低(p < 0.001)， CB2受体表达明显增加(p < 0.05)。然而，SR144528 (2mg /kg)的给药使0.3 mg/kg的舒马曲坦的所有保护作用无效。结论:我们发现CB2受体作为一种新的作用机制，在舒马曲坦对皮瓣存活的有利作用中起着至关重要的作用。因此，靶向这些受体似乎是皮瓣手术中确保其存活和防止组织坏死的可靠方法。为保证该方法的安全临床应用，还需进一步的实验和临床研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Surgical Research 医学-外科

CiteScore

2.30

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： ''European Surgical Research'' features original clinical and experimental papers, condensed reviews of new knowledge relevant to surgical research, and short technical notes serving the information needs of investigators in various fields of operative medicine. Coverage includes surgery, surgical pathophysiology, drug usage, and new surgical techniques. Special consideration is given to information on the use of animal models, physiological and biological methods as well as biophysical measuring and recording systems. The journal is of particular value for workers interested in pathophysiologic concepts, new techniques and in how these can be introduced into clinical work or applied when critical decisions are made concerning the use of new procedures or drugs.