Colloidal delivery of vitamin E into solid lipid nanoparticles as a potential complement for the adverse effects of anemia treatment

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry and Physics of Lipids Pub Date : 2022-11-01 DOI:10.1016/j.chemphyslip.2022.105252

Rocío C. Gambaro , Ignacio Rivero Berti , Maximiliano L. Cacicedo , Stephan Gehring , Vera A. Alvarez , Guillermo R. Castro , Analía Seoane , Gisel Padula , German A. Islan

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Abstract

Vitamin E (VitE) is one of the most important antioxidants and plays a key role in decreasing the inflammatory effects of oxidative stress caused by recurrent doses of iron administration in anemia treatment. However, VitE is poorly soluble in aqueous environments. Here, VitE encapsulation into solid lipid nanoparticles (SLN) composed of myristil myristate to improve its bioavailability was proposed. A 99.9 ± 0.1% encapsulation efficiency with a drug/lipid ratio of 500 µg/mg and 478 higher VitE solubility was obtained. The antioxidant properties of VitE after encapsulation were maintained. SLN-VitE showed a 228.2 nm mean diameter with low polidispersitivity (0.335), and negative Z potential (ζ ≈ −9.0 mV). The SLN were well-dispersed, displayed spherical and homogeneous morphology by TEM. A controlled release of VitE from SLN was found. The XRD and FTIR analyses revealed the presence of a nanostructured architecture of SLN after VitE incorporation. We probed the safety of SLN-VitE after contact with three in vitro cell models: erythrocytes, lymphocytes and HepG2 cells. The cell viability in presence of SLN, SLN-VitE, and their combinations with iron was not affected. The comet assay demonstrated that the DNA damage caused by iron administration was decrease in presence of SLN-VitE.

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将维生素E胶体递送到固体脂质纳米颗粒中，作为贫血治疗不良反应的潜在补充

维生素E (VitE)是最重要的抗氧化剂之一，在降低贫血治疗中反复给铁引起的氧化应激炎症反应中起关键作用。然而，VitE在水环境中难溶。本研究提出将维生素e包封在肉豆蔻酸肉豆蔻酯组成的固体脂质纳米颗粒(SLN)中，以提高其生物利用度。包封率为99.9±0.1%，药脂比为500µg/mg, VitE溶解度提高478。VitE包封后的抗氧化性能保持不变。SLN-VitE的平均直径为228.2 nm，多色散系数低(0.335)，Z电位为负(ζ≈−9.0 mV)。透射电镜显示，SLN分散良好，形貌呈球状、均匀。从SLN中发现了VitE的控释。XRD和FTIR分析表明，掺入VitE后的SLN具有纳米结构。我们通过与红细胞、淋巴细胞和HepG2细胞三种体外细胞模型接触，探讨了SLN-VitE的安全性。SLN、SLN- vite及其与铁的结合均不影响细胞活力。彗星试验表明，SLN-VitE的存在减少了铁给药引起的DNA损伤。

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来源期刊

Chemistry and Physics of Lipids 生物-生化与分子生物学

CiteScore

7.60

自引率

2.90%

发文量

审稿时长

40 days

期刊介绍： Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.