Impedance Imaging of Cells and Tissues: Design and Applications.

IF 5 Q1 ENGINEERING, BIOMEDICAL
Raziyeh Bounik, Fernando Cardes, Hasan Ulusan, Mario M Modena, Andreas Hierlemann
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引用次数: 11

Abstract

Due to their label-free and noninvasive nature, impedance measurements have attracted increasing interest in biological research. Advances in microfabrication and integrated-circuit technology have opened a route to using large-scale microelectrode arrays for real-time, high-spatiotemporal-resolution impedance measurements of biological samples. In this review, we discuss different methods and applications of measuring impedance for cell and tissue analysis with a focus on impedance imaging with microelectrode arrays in in vitro applications. We first introduce how electrode configurations and the frequency range of the impedance analysis determine the information that can be extracted. We then delve into relevant circuit topologies that can be used to implement impedance measurements and their characteristic features, such as resolution and data-acquisition time. Afterwards, we detail design considerations for the implementation of new impedance-imaging devices. We conclude by discussing future fields of application of impedance imaging in biomedical research, in particular applications where optical imaging is not possible, such as monitoring of ex vivo tissue slices or microelectrode-based brain implants.

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Abstract Image

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细胞和组织的阻抗成像:设计和应用。
由于其无标签和非侵入性,阻抗测量在生物学研究中引起了越来越多的兴趣。微制造和集成电路技术的进步为使用大规模微电极阵列对生物样品进行实时、高时空分辨率的阻抗测量开辟了一条途径。在这篇综述中,我们讨论了在细胞和组织分析中测量阻抗的不同方法和应用,重点是微电极阵列在体外应用中的阻抗成像。我们首先介绍了电极配置和阻抗分析的频率范围如何确定可以提取的信息。然后,我们深入研究了可用于实现阻抗测量的相关电路拓扑结构及其特征,如分辨率和数据采集时间。然后,我们详细介绍了实现新型阻抗成像器件的设计注意事项。最后,我们讨论了阻抗成像在生物医学研究中的未来应用领域,特别是光学成像不可能的应用,如体外组织切片或微电极脑植入物的监测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.10
自引率
0.00%
发文量
0
审稿时长
16 weeks
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