Protocadherin B9 Is Associated with Human Esophageal Squamous Cell Carcinoma Progression.

IF 3.5 4区医学 Q3 CELL BIOLOGY

Pathobiology Pub Date : 2023-01-01 DOI:10.1159/000523817

Yuto Fujiki, Akira Ishikawa, Shintaro Akabane, Shoichiro Mukai, Ryota Maruyama, Yuji Yamamoto, Aya Kido, Narutaka Katsuya, Daiki Taniyama, Kazuhiro Sentani, Naohide Oue, Wataru Yasui

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引用次数: 1

Abstract

Introduction: Esophageal cancer is the sixth leading cause of cancer-related death worldwide. However, molecular targeted therapy and novel therapeutic targets are needed for esophageal squamous cell cancer (ESCC). In a previous study, we reported that protocadherin (PCDH) B9 plays an important role in several cancers. Therefore, in this study, we examined the clinical significance of PCDHB9 expression in ESCC.

Methods: PCDHB9 expression was examined using immunohistochemistry in 128 cases and using quantitative reverse transcription-polymerase chain reaction in 16 cases of ESCC. PCDHB9 function in ESCC cells was examined using RNA interference.

Results: High PCDHB9 expression was identified in 5 of 16 (31.3%). In total, 51 (40%) ESCC cases showed strong PCDHB9 expression, whereas nonneoplastic mucosa rarely showed its expression. High PCDHB9 expression was significantly associated with T classification, N grade, and stage in ESCC. In ESCC cell lines, PCDHB9 knockdown affected cell growth, migration, and adhesion. Further, the expression of integrin (ITG) A3, ITGA4, ITGA5, ITGB1, ITGB6, vimentin, snail family transcriptional repressor 1, and cadherin 2 (NCAD) was significantly reduced and cadherin 1 was significantly increased in PCDHB9 knockdown ESCC cells.

Conclusion: These results suggest that PCDHB9 plays a tumor-promoting role and is a potential biomarker and therapeutic target in ESCC.

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原钙粘蛋白B9与人食管鳞状细胞癌进展相关

食管癌是全球癌症相关死亡的第六大原因。然而，食管鳞状细胞癌(ESCC)需要分子靶向治疗和新的治疗靶点。在之前的研究中，我们报道了原钙粘蛋白(PCDH) B9在几种癌症中起重要作用。因此，在本研究中，我们探讨了PCDHB9在ESCC中的表达的临床意义。方法:128例ESCC采用免疫组化法检测PCDHB9表达，16例ESCC采用定量逆转录聚合酶链反应法检测PCDHB9表达。采用RNA干扰法检测PCDHB9在ESCC细胞中的功能。结果:16例患者中有5例(31.3%)高表达PCDHB9。51例(40%)ESCC中PCDHB9表达强烈，而非肿瘤粘膜很少表达。PCDHB9高表达与ESCC的T分型、N分级和分期有显著相关性。在ESCC细胞系中，PCDHB9敲低影响细胞生长、迁移和粘附。此外，在PCDHB9敲低的ESCC细胞中，整合素(ITG) A3、ITGA4、ITGA5、ITGB1、ITGB6、vimentin、蜗牛家族转录抑制因子1和cadherin 2 (NCAD)的表达显著降低，cadherin 1的表达显著升高。结论:PCDHB9具有促肿瘤作用，是ESCC潜在的生物标志物和治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathobiology 医学-病理学

CiteScore

8.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： ''Pathobiology'' offers a valuable platform for the publication of high-quality original research into the mechanisms underlying human disease. Aiming to serve as a bridge between basic biomedical research and clinical medicine, the journal welcomes articles from scientific areas such as pathology, oncology, anatomy, virology, internal medicine, surgery, cell and molecular biology, and immunology. Published bimonthly, ''Pathobiology'' features original research papers and reviews on translational research. The journal offers the possibility to publish proceedings of meetings dedicated to one particular topic.

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