Correlation of the DNA Concentration of Human Samples to Electrical Bioimpedance Measurements: A Pilot Study.

Q3 Biochemistry, Genetics and Molecular Biology

Journal of Electrical Bioimpedance Pub Date : 2022-01-01 DOI:10.2478/joeb-2022-0018

César A Hernández-Salinas, Alejandro Corzo-Cruz, Virginia Sánchez-Monroy, Jacobo E Munguía-Cervantes, César A González-Díaz

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Abstract

It is necessary to evaluate the total deoxyribonucleic acid (DNA) concentration in gene expression assays. The existing techniques require equipment that is expensive for many labs in developing countries. Portable and inexpensive equipment is needed for easy and economical DNA quantification. Electrical bioimpedance spectroscopy (EBiS) is a non-invasive and inexpensive technique for examining the electrical properties of biological materials. The aim of this study was to explore a potential correlation between the measurement of total DNA extracted from human samples by UV-Vis spectrophotometry and EBiS. Hence, after quantifying the total DNA extracted from each sample by UV-Vis spectroscopy, EBiS was recorded and a possible correlation between the two measurements was analyzed. Considering the bioimpedance phase parameter at 5.24 MHz, a significant correlation was found with total DNA, especially when the concentration was below 100 ng/μL (Spearman coefficient = 0.82, p<0.005). Additional experiments are warranted to confirm these findings.

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人体样本DNA浓度与电生物阻抗测量的相关性:一项初步研究。

在基因表达分析中，总脱氧核糖核酸(DNA)浓度的测定是必要的。现有技术需要的设备对发展中国家的许多实验室来说都很昂贵。简便、经济的DNA定量需要便携、廉价的设备。生物阻抗谱(EBiS)是一种非侵入性和廉价的技术，用于检查生物材料的电学特性。本研究的目的是探讨紫外-可见分光光度法测定人体样品中提取的总DNA与ebi之间的潜在相关性。因此，在用紫外-可见光谱定量每个样品中提取的总DNA后，记录ebi并分析两者之间可能的相关性。在5.24 MHz时，生物阻抗相位参数与总DNA呈极显著相关，特别是当浓度低于100 ng/μL时(Spearman系数= 0.82,p

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