An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development.

IF 45.5 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cell Pub Date : 2023-09-28 Epub Date: 2023-09-12 DOI:10.1016/j.cell.2023.08.019

Zeshuai Wang, Zhisheng Wu, Hao Wang, Ruoqing Feng, Guanlin Wang, Muxi Li, Shuang-Yin Wang, Xiaoyan Chen, Yiyi Su, Jun Wang, Weiwen Zhang, Yuzhou Bao, Zhenwei Lan, Zhuo Song, Yiheng Wang, Xianyang Luo, Lingyu Zhao, Anli Hou, Shuye Tian, Hongliang Gao, Wenbin Miao, Yingyu Liu, Huilin Wang, Cui Yin, Zhi-Liang Ji, Mingqian Feng, Hongkun Liu, Lianghui Diao, Ido Amit, Yun Chen, Yong Zeng, Florent Ginhoux, Xueqing Wu, Yuanfang Zhu, Hanjie Li

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引用次数: 0

Abstract

Macrophages are heterogeneous and play critical roles in development and disease, but their diversity, function, and specification remain inadequately understood during human development. We generated a single-cell RNA sequencing map of the dynamics of human macrophage specification from PCW 4-26 across 19 tissues. We identified a microglia-like population and a proangiogenic population in 15 macrophage subtypes. Microglia-like cells, molecularly and morphologically similar to microglia in the CNS, are present in the fetal epidermis, testicle, and heart. They are the major immune population in the early epidermis, exhibit a polarized distribution along the dorsal-lateral-ventral axis, and interact with neural crest cells, modulating their differentiation along the melanocyte lineage. Through spatial and differentiation trajectory analysis, we also showed that proangiogenic macrophages are perivascular across fetal organs and likely yolk-sac-derived as microglia. Our study provides a comprehensive map of the heterogeneity and developmental dynamics of human macrophages and unravels their diverse functions during development.

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免疫细胞图谱揭示了人类巨噬细胞在产前发育过程中的动态特性。

巨噬细胞是异质性的，在发育和疾病中发挥着关键作用，但在人类发育过程中，对其多样性、功能和特性的了解仍然不足。我们生成了19个组织中PCW 4-26的人类巨噬细胞规格动态的单细胞RNA测序图。我们在15种巨噬细胞亚型中确定了一个小胶质细胞样群体和一个促血管生成群体。小胶质细胞样细胞在分子和形态上与中枢神经系统中的小胶质细胞相似，存在于胎儿表皮、睾丸和心脏中。它们是早期表皮中的主要免疫群体，沿背侧-腹侧轴呈极化分布，并与神经嵴细胞相互作用，调节其沿黑素细胞谱系的分化。通过空间和分化轨迹分析，我们还表明，促血管生成巨噬细胞是胎儿器官的血管周巨噬细胞，可能是来源于小胶质细胞的卵黄囊。我们的研究提供了人类巨噬细胞异质性和发育动力学的全面图谱，并揭示了它们在发育过程中的不同功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell 生物-生化与分子生物学

CiteScore

110.00

自引率

0.80%

发文量

396

审稿时长

2 months

期刊介绍： Cells is an international, peer-reviewed, open access journal that focuses on cell biology, molecular biology, and biophysics. It is affiliated with several societies, including the Spanish Society for Biochemistry and Molecular Biology (SEBBM), Nordic Autophagy Society (NAS), Spanish Society of Hematology and Hemotherapy (SEHH), and Society for Regenerative Medicine (Russian Federation) (RPO). The journal publishes research findings of significant importance in various areas of experimental biology, such as cell biology, molecular biology, neuroscience, immunology, virology, microbiology, cancer, human genetics, systems biology, signaling, and disease mechanisms and therapeutics. The primary criterion for considering papers is whether the results contribute to significant conceptual advances or raise thought-provoking questions and hypotheses related to interesting and important biological inquiries. In addition to primary research articles presented in four formats, Cells also features review and opinion articles in its "leading edge" section, discussing recent research advancements and topics of interest to its wide readership.