Microbial degradation of the benzimidazole fungicide carbendazim by Bacillus velezensis HY-3479.

Abstract

Carbendazim (methyl benzimidazol-2-ylcarbamate: MBC) is a fungicide of the benzimidazole group that is widely used in the cultivation of pepper, ginseng, and many other crops. To remove the remnant carbendazim, many rhizobacteria are used as biodegradation agents. A bacterial strain of soil-isolated Bacillus velezensis HY-3479 was found to be capable of degrading MBC in M9 minimal medium supplemented with 250 mg/L carbendazim. The strain had a significantly higher degradation efficiency compared to the control strain Bacillus subtilis KACC 15590 in high-performance liquid chromatography (HPLC) analysis, and HY-3479 had the best degradation efficiency of 76.99% at 48 h. In gene expression analysis, upregulation of carbendazim-degrading genes (mheI, hdx) was observed in the strain. HY-3479 was able to use MBC as the sole source of carbon and nitrogen, but the addition of 12.5 mM NH₄NO₃ significantly increased the degradation efficiency. HPLC analysis showed that the degradation efficiency increased to 87.19% when NH₄NO₃ was added. Relative gene expression of mheI and hdx also increased for samples with NH₄NO₃ supplementation. The enzyme activity of the carbendazim-degrading enzyme and the 2-aminobenzimidazole-degrading enzyme was found to be highly present in the HY-3479 strain. It is the first reported B. velezensis strain to biodegrade carbendazim (MBC). The biodegradation activity of strain HY-3479 may be developed as a useful means for bioremediation and used as a potential microbial agent in sustainable agriculture.