CRISPR-based diagnostics

IF 26.8 1区医学 Q1 ENGINEERING, BIOMEDICAL

Nature Biomedical Engineering Pub Date : 2021-07-16 DOI:10.1038/s41551-021-00760-7

Michael M. Kaminski, Omar O. Abudayyeh, Jonathan S. Gootenberg, Feng Zhang, James J. Collins

引用次数: 278

Abstract

The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic acids are among the most sensitive and specific, yet most such assays require costly equipment and trained personnel. Recent developments in diagnostic technologies, in particular those leveraging clustered regularly interspaced short palindromic repeats (CRISPR), aim to enable accurate testing at home, at the point of care and in the field. In this Review, we provide a rundown of the rapidly expanding toolbox for CRISPR-based diagnostics, in particular the various assays, preamplification strategies and readouts, and highlight their main applications in the sensing of a wide range of molecular targets relevant to human health. This Review summarizes the rapidly expanding toolbox of assays for CRISPR-based diagnostics and highlights their point-of-care applications.

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基于 CRISPR 的诊断

准确及时的疾病诊断是高效治疗干预和流行病学监测的先决条件。基于核酸检测的诊断方法是最灵敏、最特异的诊断方法之一，但大多数此类检测方法都需要昂贵的设备和训练有素的人员。诊断技术的最新发展，特别是利用成簇的有规则间隔短回文重复序列（CRISPR）的诊断技术，旨在实现家庭、医疗点和现场的精确检测。在本综述中，我们简要介绍了迅速扩展的基于 CRISPR 的诊断工具箱，特别是各种检测方法、前置扩增策略和读数，并重点介绍了它们在感知与人类健康相关的各种分子靶标方面的主要应用。本综述总结了基于CRISPR技术的诊断工具箱的快速发展，并重点介绍了其在护理点的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Biomedical Engineering Medicine-Medicine (miscellaneous)

CiteScore

45.30

自引率

1.10%

发文量

138

期刊介绍： Nature Biomedical Engineering is an online-only monthly journal that was launched in January 2017. It aims to publish original research, reviews, and commentary focusing on applied biomedicine and health technology. The journal targets a diverse audience, including life scientists who are involved in developing experimental or computational systems and methods to enhance our understanding of human physiology. It also covers biomedical researchers and engineers who are engaged in designing or optimizing therapies, assays, devices, or procedures for diagnosing or treating diseases. Additionally, clinicians, who make use of research outputs to evaluate patient health or administer therapy in various clinical settings and healthcare contexts, are also part of the target audience.