Aqueous extract of Achillea millefolium significantly affects mesenchymal stem cell renewal and differentiation in a dose dependent manner.

IF 1.1 Q4 CELL & TISSUE ENGINEERING

Journal of Stem Cells & Regenerative Medicine Pub Date : 2022-01-01 DOI:10.46582/jsrm.1802006

Fatemeh Asadi, Fatemeh Ayoobi, Mohammad Kazemi Arababadi, Zeinab Kazemi Arababadi, Aliakbar Yousefi-Ahmadipour

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引用次数: 1

Abstract

Introduction: Achillea millefolium is an Iranian herbal medicine with various effects on the human cells. The aim of this study was to investigate the effects of the aqueous extract of Achillea millefolium (AEAM) on the proliferation and differentiation of mesenchymal stem cells (MSCs).

Methods: In this study, bone marrow-MSCs (BM-MSCs) were obtained from Wister rat bone morrow and then cultured in Dulbecco's modified Eagle's medium /Nutrient Ham's Mixture F-12 (DMEM/F12) media. Then, the isolated MSCs were cultured in either osteocyte or adipocyte differentiation media containing 0.2 or 2 mg/mL AEAM and assessed using specific staining method.

Results: The isolated BM-MSCs exhibited fibroblast-like morphology and were positive for CD73, and CD90, while negative for CD34 and CD45. AEAM significantly increased self-renewal of BM-MSCs at low dose (0.2 mg/ml, P= 0.001) and increased the pool stem cells in both osteocyte and adipocyte differentiation media.

Conclusion: AEAM at low doses may be used in cases where there is a need for large number of stem cells, via increased numbers of MSCs, and help tissue repair and immunomodulation.

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千叶跟头水提物对间充质干细胞的更新和分化具有剂量依赖性。

千叶阿喀琉叶是一种伊朗草药，对人体细胞有多种作用。本研究旨在探讨千叶阿喀琉叶水提物(aam)对间充质干细胞(MSCs)增殖和分化的影响。方法:从Wister大鼠骨中提取骨髓间充质干细胞(BM-MSCs)，在Dulbecco's modified Eagle's medium / nutrition Ham's Mixture F-12 (DMEM/F12)培养基中培养。然后，将分离的MSCs在含有0.2或2 mg/mL AEAM的骨细胞或脂肪细胞分化培养基中培养，并采用特异性染色方法进行评估。结果:BM-MSCs呈成纤维细胞样形态，CD73、CD90表达阳性，CD34、CD45表达阴性。AEAM在低剂量(0.2 mg/ml, P= 0.001)下显著增加了BM-MSCs的自我更新，并增加了骨细胞和脂肪细胞分化培养基中的池干细胞。结论:低剂量的AEAM可用于需要大量干细胞的病例，通过增加MSCs的数量，有助于组织修复和免疫调节。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Stem Cells & Regenerative Medicine CELL & TISSUE ENGINEERING-

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

14 weeks